gms | German Medical Science

56. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie e. V. (DGNC)
3èmes journées françaises de Neurochirurgie (SFNC)

Deutsche Gesellschaft für Neurochirurgie e. V.
Société Française de Neurochirurgie

07. bis 11.05.2005, Strasbourg

Lack of toxicity of oncolytic Parvovirus H-1 in untransformed adult human brain cells

Parvovirus H-1: Onkoselektivität im humanen Hirngewebe

Meeting Abstract

  • corresponding author M. Herrero y Calle - Neurochirurgische Universitätsklinik, Freiburg
  • J. Cornelis - Deutsches Krebsforschungszentrum, ATV, Heidelberg
  • C. Sommer - Abteilung für Neuropathologie, Universitätsklinikum Ulm
  • J. R. Schlehofer - Deutsches Krebsforschungszentrum, ATV, Heidelberg
  • J. Rommelaere - Deutsches Krebsforschungszentrum, ATV, Heidelberg
  • K. Geletneky - Neurochirurgische Universitätsklinik, Heidelberg

Deutsche Gesellschaft für Neurochirurgie. Société Française de Neurochirurgie. 56. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie e.V. (DGNC), 3èmes journées françaises de Neurochirurgie (SFNC). Strasbourg, 07.-11.05.2005. Düsseldorf, Köln: German Medical Science; 2005. DocP170

The electronic version of this article is the complete one and can be found online at:

Published: May 4, 2005

© 2005 Herrero y Calle et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.




We already proved the efficient oncolytic effect of Parvovirus H-1 in human glioblastoma cell lines as well as in short-term cultures of glioblastomas and one gliosarcoma. For a variety of human cells but not for normal glial cells, Parvovirus H-1 is known to show limited-to-no cytocidal activity. In this study we report about the effects of H-1 virus infection on non-transformed human glial short-term cultures as well as on adult human organotypic brain cultures.


Specimens of adult human brain were obtained during epilepsy surgery. Isolation, culture and identification of glial cells was performed following the protocol of Brewer et al. 2001. Organotypic brain cultures were obtained following the method of Jung et al. 2001. Monolayers of glial cell cultures were inoculated with H-1 virus. Growth curves were recorded from infected and mock-treated cells. A qualitative analysis of the biosynthesis of all viral macromolecular components was performed. Organotypic cultures were inoculated with H-1 virus. Infected and mock-treated cultures were harvested on day 1,2,3,4,5 after infection and fixed in 4% PFA for histological examination.


We were able to detect a synthesis of all viral macromolecular components in glial short-term cultures but there was no efficient cytolytic effect of H-1 virus infection. Growth curves of infected and mock-treated cultures showed no differences at a multiplicity of infection (MOI) of 1 plaque forming unit (pfu)/cell. At a MOI of 10 pfu/cell the infected cultures showed growth arrest in comparison to their mock-treated counterparts, but no altered morphology or cell lysis, maintaining an equal number of cells on day 1,3 and 6 after infection compared to the initial cell count. The histological examination of the organotypic cultures did not show signs of tissue damage on day 1,2,3,4 and 5 after infection with H-1 virus.


Infection of adult glial short-term cultures with parvovirus H-1 leads to viral replication but not to cytotoxic effects. Furthermore, infection of organotypic adult human brain cultures did not reveal any signs of tissue damage. This data supports the oncoselectivity of Parvovirus H-1, making it a promising candidate for oncolytic viral therapy of gliomas.