gms | German Medical Science

56. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie e. V. (DGNC)
3èmes journées françaises de Neurochirurgie (SFNC)

Deutsche Gesellschaft für Neurochirurgie e. V.
Société Française de Neurochirurgie

07. bis 11.05.2005, Strasbourg

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Vascular endothelial growth factor (VEGF) is significantly involved in glioma-induced migration of adult human mesenchymal stem cells

VEGF ist ein signifikanter Faktor der Gliom-induzierten Migration adulter mesenchymaler Stammzellen

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  • corresponding author C. Schichor - Neurosurgical Clinic Grosshadern, Ludwig-Maximilians-University, Munich
  • T. Birnbaum - Neurological Clinic Grosshadern, Ludwig-Maximilians-University, Munich
  • C. Padovan - Neurological Clinic Grosshadern, Ludwig-Maximilians-University, Munich
  • O. Schnell - Neurosurgical Clinic Grosshadern, Ludwig-Maximilians-University, Munich
  • S. Lange - Neurosurgical Clinic Grosshadern, Ludwig-Maximilians-University, Munich
  • S. Grau - Neurosurgical Clinic Grosshadern, Ludwig-Maximilians-University, Munich
  • S. Miebach - Neurosurgical Clinic Grosshadern, Ludwig-Maximilians-University, Munich
  • N. Etminan - Neurosurgical Clinic Grosshadern, Ludwig-Maximilians-University, Munich
  • J.-C. Tonn - Neurosurgical Clinic Grosshadern, Ludwig-Maximilians-University, Munich
  • R. Goldbrunner - Neurosurgical Clinic Grosshadern, Ludwig-Maximilians-University, Munich

Deutsche Gesellschaft für Neurochirurgie. Société Française de Neurochirurgie. 56. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie e.V. (DGNC), 3èmes journées françaises de Neurochirurgie (SFNC). Strasbourg, 07.-11.05.2005. Düsseldorf, Köln: German Medical Science; 2005. Doc10.05.-08.03

The electronic version of this article is the complete one and can be found online at: http://www.egms.de/en/meetings/dgnc2005/05dgnc0122.shtml

Published: May 4, 2005

© 2005 Schichor et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.en). You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.

Outline

Text

Objective

Much effort has been put into establishing human multipotent cells as carriers for malignant glioma therapy. We already described interaction of adult human mesenchymal stem cells (hMSC), which are easily available through bone marrow biopsy and glioma cells in vitro. Aim of our study was to characterise glioma-induced, VEGF-dependent migratory and invasive MSC- behaviour.

Methods

Human MSC were isolated from bone marrow biopsies carried out for hematological indications. Only early passages were used for the experiments. Chemokinetic activity of hMSC in response to glioma-conditioned medium as well as VEGF was evaluated using a modified Boyden chamber assay. Confrontational co-cultures of glioma- (U373 GFP, C6 GFP, C6VEGF sense, C6 VEGF antisense transfected) and stem cell-spheroids (hMSC) were investigated. Invasion was visualised by light and confocal microscopy. VEGF-secretion by gliomas was analysed by ELISA. Expression of VEGF-receptor flk-1 in hMSCs was assessed immunohistochemically.

Results

ELISA-analysis showed high VEGF-expression in all glioma cell lines. VEGF as well as tumour-conditioned medium significantly increased hMSC migration and invasion. hMSC showed an extensive invasion into glioma spheroids. Addition of anti-human VEGF antibody significantly inhibited the VEGF-dependent effect of glioma-driven hMSC-invasion.

Conclusions

hMSC show intensive migratory and invasive behaviour in presence of glioma cells and glioma-conditioned medium. Obviously, VEGF is a crucial factor in enhancing stem cell motility. hMSC proved to be hopeful candidates for a future role as glioma treatment vectors.