gms | German Medical Science

29. Wissenschaftlicher Kongress der Deutschen Hochdruckliga

Deutsche Hochdruckliga e. V. DHL ® - Deutsche Hypertonie Gesellschaft Deutsches Kompetenzzentrum Bluthochdruck

23. bis 25.11.2005, Berlin

Increased transient receptor potential channels in patients with essential hypertension

Steigerung von Transient Rezeptor Potential Kanälen bei Patienten mit essentieller Hypertonie

Meeting Abstract

Suche in Medline nach

  • M. Tepel - Charité Campus Benjamin Franklin, (Berlin, D)
  • F. Thilo - Charité Campus Benjamin Franklin, (Berlin, D)
  • A. Scholze - Charité Campus Benjamin Franklin, (Berlin, D)
  • D. Liu - Charité Campus Benjamin Franklin, (Berlin, D)
  • W. Zidek - Charité Campus Benjamin Franklin, (Berlin, D)

Hypertonie 2005. 29. Wissenschaftlicher Kongress der Deutschen Hochdruckliga. Berlin, 23.-25.11.2005. Düsseldorf, Köln: German Medical Science; 2006. Doc05hochP19

Die elektronische Version dieses Artikels ist vollständig und ist verfügbar unter: http://www.egms.de/de/meetings/hoch2005/05hoch019.shtml

Veröffentlicht: 8. August 2006

© 2006 Tepel et al.
Dieser Artikel ist ein Open Access-Artikel und steht unter den Creative Commons Lizenzbedingungen (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.de). Er darf vervielf&aauml;ltigt, verbreitet und &oauml;ffentlich zug&aauml;nglich gemacht werden, vorausgesetzt dass Autor und Quelle genannt werden.


Gliederung

Text

Alterations of cellular cation influx have frequently been described in essential hypertension. However, the role of non-selective cation channels of the transient receptor potential (TRP) family in hypertension has not been evaluated yet. We investigated TRPC3, TRPC5, TRPC6 in human monocytes from normotensive control subjecs and age- and sex-matched patients with essential hypertension using real-time polymerase chain reaction and in-cell Western assay. Quantitiative TRPC mRNA measurements were normalized to GAPDH, and TRPC channel protein expression was normalized to CD11a as internal reference. Cation influx was measured using the fluorescent dye technique and quantified as fluorescence increase above baseline. The normalized expression of TRPC mRNA was significantly higher in patients with essential hypertension compared to normotensive control subjects (1.492±0.028, n=27; vs 1.515±0.022, n=23; mean±SEM; p<0.05 by Mann-Whitney-Test). Using in-cell Western assay, we observed a significant increase of TRPC3 and TRPC5 channel protein expression in cells from essential hypertensive patients compared to normotensive control subjects (normalized TRPC3 expression, 3.21±0.59 vs. 1.36±0.07; p<0.01; normalized TRPC5 expression, 2.10±0.28 vs. 1.40±0.52; n=31; p<0.01 by Mann-Whitney-Test). Gadolinium influx was significantly higher in patients with essential hypertension compared to normotensive control subjects (48±4% vs. 36±3%; each n=51; p<0.05 by Mann-Whitney-Test). The study for the first time shows increased TRPC3 and TRPC5 expression in essential hypertension