Artikel
Excessive hypertension and cardiac hypertrophy in transgenic mice carrying the rat angiotensinogene gene
Extremer Bluthochdruck und kardiale Hypertrophie in transgenen Mäusen, die das Angiotensinogen Gen von Ratten tragen
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Autoren
Veröffentlicht: | 11. November 2004 |
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Gliederung
Text
Transgenic mice were generated by injecting the rat angiotensinogen gene into the germline of NMRI mice. The resulting transgenic line TGM(rAOGEN)123 was characterized (Kimura et al., 1992). The aim of our study was to characterize a second generated transgenic line TGM(rAOGEN)102.
Methods
Seven transgenic animals and 6 wild-type animals (1 year old) have been identified by PCR. Blood pressure measurement was performed using an invasive catheter into the carotid artery. Hearts were cut and the ratio of ventricle and body weight was calculated. RNase-protection assay for collagen III was performed using ventricular RNA.
Results
All transgenic mice had a significantly elevated systolic blood pressure (179±7.5 mmHg) compared with control mice (130±6.7 mmHg) (p<0.001), whereby 3 animals reached levels of more than 190 mmHg. The rise of blood pressure has been paralleled by a cardiac hypertrophy, (ventricle/body weight ratio: L102:4.2±0.25 mg/g, control 2.8±0.04 mg/g, p<0.001). However collagen III RNA was not significantly increased in the heart of transgenic mice.
Conclusions
Our data indicate that overexpression of angiotensinogen leads to excessive hypertension. Thus, the line TGM(rAOGEN)102 provides a new model to study hypertension-mediated end organ damage and the potency of antihypertensive drugs.