gms | German Medical Science

27. Wissenschaftlicher Kongress der Deutschen Hochdruckliga

Deutsche Liga zur Bekämpfung des hohen Blutdrucks – Deutsche Hypertonie Gesellschaft e. V.

26. bis 29.11.2003, Bonn

Excessive hypertension and cardiac hypertrophy in transgenic mice carrying the rat angiotensinogene gene

Extremer Bluthochdruck und kardiale Hypertrophie in transgenen Mäusen, die das Angiotensinogen Gen von Ratten tragen

Meeting Abstract (Hypertonie 2003)

  • presenting/speaker P. Xu - Universitätsklinikum Benjamin Franklin, Max-Delbrück Center (Berlin, D)
  • M. Bader - Universitätsklinikum Benjamin Franklin, Max-Delbrück Center (Berlin, D)
  • H.P. Schultheiss - Universitätsklinikum Benjamin Franklin, Max-Delbrück Center (Berlin, D)
  • T. Walther - Universitätsklinikum Benjamin Franklin, Max-Delbrück Center (Berlin, D)

Hypertonie 2003. 27. Wissenschaftlicher Kongress der Deutschen Hochdruckliga. Bonn, 26.-29.11.2003. Düsseldorf, Köln: German Medical Science; 2004. Doc03hochV18

The electronic version of this article is the complete one and can be found online at: http://www.egms.de/en/meetings/hoch2003/03hoch018.shtml

Published: November 11, 2004

© 2004 Xu et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.en). You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.


Outline

Text

Transgenic mice were generated by injecting the rat angiotensinogen gene into the germline of NMRI mice. The resulting transgenic line TGM(rAOGEN)123 was characterized (Kimura et al., 1992). The aim of our study was to characterize a second generated transgenic line TGM(rAOGEN)102.

Methods

Seven transgenic animals and 6 wild-type animals (1 year old) have been identified by PCR. Blood pressure measurement was performed using an invasive catheter into the carotid artery. Hearts were cut and the ratio of ventricle and body weight was calculated. RNase-protection assay for collagen III was performed using ventricular RNA.

Results

All transgenic mice had a significantly elevated systolic blood pressure (179±7.5 mmHg) compared with control mice (130±6.7 mmHg) (p<0.001), whereby 3 animals reached levels of more than 190 mmHg. The rise of blood pressure has been paralleled by a cardiac hypertrophy, (ventricle/body weight ratio: L102:4.2±0.25 mg/g, control 2.8±0.04 mg/g, p<0.001). However collagen III RNA was not significantly increased in the heart of transgenic mice.

Conclusions

Our data indicate that overexpression of angiotensinogen leads to excessive hypertension. Thus, the line TGM(rAOGEN)102 provides a new model to study hypertension-mediated end organ damage and the potency of antihypertensive drugs.