gms | German Medical Science

83. Jahresversammlung der Deutschen Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie e. V.

Deutsche Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie e. V.

16.05. - 20.05.2012, Mainz

Influence of superparamagnetic iron oxide nanoparticles (VSOP) on differentiation, proliferation, vitality and genetic stability of mesenchymal stem cells

Meeting Abstract

German Society of Oto-Rhino-Laryngology, Head and Neck Surgery. 83rd Annual Meeting of the German Society of Oto-Rhino-Laryngology, Head and Neck Surgery. Mainz, 16.-20.05.2012. Düsseldorf: German Medical Science GMS Publishing House; 2012. Doc12hno69

DOI: 10.3205/12hno69, URN: urn:nbn:de:0183-12hno693

Veröffentlicht: 23. Juli 2012

© 2012 Ramos Tirado et al.
Dieser Artikel ist ein Open Access-Artikel und steht unter den Creative Commons Lizenzbedingungen (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.de). Er darf vervielfältigt, verbreitet und öffentlich zugänglich gemacht werden, vorausgesetzt dass Autor und Quelle genannt werden.


Gliederung

Text

Introduction: Tissue engineering with adipose tissue derived stem cells (ASC) can be used for the generation of transplants in reconstructive surgery. The labeling of ASC with superparamagnetic iron oxide nanoparticles allows tracking of the cells over large periods of time by the means of MRI. The aims of the investigation were the evaluation of genotoxic and cytotoxic effects as well as influences on differentiation, proliferation and vitality of ASC by VSOP.

Methods: After isolation of ASC from human liposuction material of 8 patients and the subsequent proliferation of the cells up to the passages 2 to 4, ASC were labeled with 4 different VSOP concentrations (1,5 µM to 1,5 mM). The influence of labeling on chondrogenic, adipogenic and osteogenic differentiation was examined histologically, biochemically and by measures of biology. Proliferation, migration, and cell vitality of the cells were analyzed. To evaluate the genotoxicity of VSOP the comet assay and chromosomal aberration test were used.

Results: Labeling with VSOP had no effect on the multi-differentiation potential of ASC. DNA fragmentation and chromosomal aberrations were mainly detected at the highest concentration and decreased at lower concentrations. Vitality and proliferation were not altered by the VSOP labeling.

Conclusions: The in vitro differentiation into various cell types of human ASC labeled with VSOP could be achieved. It was possible to determine a suitable VSOP concentration with minimal cytotoxic and genotoxic effects on ASC within the detection limit of MRI. Long-term studies on the behavior of iron labeled cells in in vivo models are suggested before its application in humans.