gms | German Medical Science

78. Jahresversammlung der Deutschen Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie e. V.

Deutsche Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie e. V.

16.05. - 20.05.2007, München

Gene expression analysis with mRNA out of natural killer cells from ENT carcinoma patients

Meeting Abstract

  • corresponding author Jürgen Ostwald - ENT department and clinic "Otto Körner", University of Rostock, Rostock, Germany
  • author Arne Böttcher - ENT department and clinic "Otto Körner", University of Rostock, Rostock, Germany
  • author Steffen Dommerich - ENT department and clinic "Otto Körner", University of Rostock, Rostock, Germany
  • author Burkhard Kramp - ENT department and clinic "Otto Körner", University of Rostock, Rostock, Germany
  • author Dirk Koczan - Institute of Immunology, University of Rostock, Rostock, Germany

German Society of Oto-Rhino-Laryngology, Head and Neck Surgery. 78th Annual Meeting of the German Society of Oto-Rhino-Laryngology, Head and Neck Surgery. Munich, 16.-20.05.2007. Düsseldorf, Köln: German Medical Science; 2007. Doc07hno087

Die elektronische Version dieses Artikels ist vollständig und ist verfügbar unter: http://www.egms.de/de/meetings/hno2007/07hno087.shtml

Veröffentlicht: 8. August 2007

© 2007 Ostwald et al.
Dieser Artikel ist ein Open Access-Artikel und steht unter den Creative Commons Lizenzbedingungen (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.de). Er darf vervielf&aauml;ltigt, verbreitet und &oauml;ffentlich zug&aauml;nglich gemacht werden, vorausgesetzt dass Autor und Quelle genannt werden.


Gliederung

Text

Introduction: Own previous research showed significantly raised amounts of natural killer (NK) cells in peripheral venous blood count on treated non-recurrent ENT carcinoma patients. In comparison to non-treated preoperative carcinoma patients and healthy probands an approximate doubling in both absolute amount of NK cells and relative share of peripheral blood lymphocytes was established (Figure 1 [Fig. 1], Figure 2 [Fig. 2]). In order to understand the regulatory mechanisms of this phenomenon the expression levels of the whole human genome on mRNA basis out of NK cells have been compared between 3 proband groups. These groups were clinically defined as: previously non-treated carcinoma patients, successfully treated non-recurrent patients and healthy probands.

Methods: Lymphocytes were prepared from peripheral blood specimen by density gradient centrifugation. Afterwards NK cells were separated and counted by using magnetic cell sorting and flowcytometry. After dissolving NK cells and isolating the whole RNA, the latter was prepared for genomewide expression profiling by the Human Genome U133 PLUS 2.0 gene chip. This technique allows to define the expression level of more than 47,000 transcripts and variants, including about 38,500 well-described human genes. 4 probands of each group were investigated. A functional gene interpretation was performed with common gene databanks.

Results: Comparing the 3 groups 27 to 114 transcripts could be identified that were diversely expressed in a decreased or increased way. A central role could be attributed to chemokine receptors CCR1, CCR7, CXCR3, cytokine XLC1 and transcription factors MYC and CAMK4 which were upregulated. Membrane protein DLL1 and NK cell receptor KLRC3/NKG2E seem to exert influence by downregulated expression (Table 1 [Tab. 1], Table 2 [Tab. 2]).

Conclusion: Knowledge in exact mechanisms of the rise of NK cell population in peripheral blood could be important as a possible strategy in immunotherapy on oncologic patients. A potency in prevention of carcinoma recurrence after surgical treatment is conceivable.


References

1.
Ostwald J, Dommerich S, Schulz U, Kramp B. Long-term changes in peripheral blood leukocyte and lymphocyte populations in ENT-carcinoma patients. A flow cytometric study in 346 ENT-carcinoma patients and 31 healthy controls. HNO. 2004.
2.
Affymetrix, Inc. Design and Performance of the GeneChip Human Genome U133 Plus 2.0 and Human Genome U133A 2.0 Arrays. Part No. 701483 Rev. 2; 2003.
3.
Becton-Dickinson Immunocytometry systems. Clinical Monograph Nr. 1. Normal values: Definition of a reference range for lymphocyte subsets of healthy adults. San Jose USA; 1991.