Artikel
Identification of deregulated functional groups on mRNA-level by microarray-technique in head and neck carcinomas
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Autoren
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Christian Cordes
- Department of Otorhinolaryngology, Head and Neck Surgery, University Hospital Schleswig-Holstein, Campus Kiel, Germany
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Stefan Gottschlich
- Department of Otorhinolaryngology, Head and Neck Surgery, University Hospital Schleswig-Holstein, Campus Kiel, Germany
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Robert Häsler
- I. Department of Internal Medicine, University Hospital Schleswig-Holstein, Campus Kiel, Germany
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Petra Ambrosch
- Department of Otorhinolaryngology, Head and Neck Surgery, University Hospital Schleswig-Holstein, Campus Kiel, Germany
| Veröffentlicht: | 22. September 2005 |
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Gliederung
Text
The exact molecular mechanismns coursing the development of squamous cell carcinomas in head and neck region are mostly unknown. Identification of genetic changes which are involved in carcinogenesis might play a key role for improvement of diagnosis, therapy and finally prognosis of patients with carcinomas in head and neck region. One method to identify those mechanismns is the so called microarray-technique. It allows to measure gene expression of hundreds of genes of one cell or of one tissue simultaneously. We measured the expression of 408 apoptosis associated genes by microarray-technique in normal upper aerodigestive tract mucosa (n=3), in cancer edge (n=2) and in cancer center (n=4) of squamous cell carcinomas of the upper aero-digestive-tract. For statistical analysis a two dimensional hierarchical clustering with statistical predefined groups was performed. 11 with antiapoptotical processes connected genes were identified which were upregulated. 15 with proapoptotic associated genes as well as 5 with DNA-replication and chromosome cycle associated genes were downregulated in tumor tissue. Furthermore 6 of 8 with lymphocyte activation associated genes were downregulated in tumor tissue. These results allowed a classification of tissue in the 3 predefined groups by 22 marker genes. These results are a first step in diagnostic usage of microarrays in squamous cell carcinomas of head and neck region and might improve the understanding of mechanismns of carcinogenesis in head and neck region.
