gms | German Medical Science

102. Jahrestagung der DOG

Deutsche Ophthalmologische Gesellschaft e. V.

23. bis 26.09.2004, Berlin

The effect of VEGF-C on secretion of VEGF-A of the RPE

Meeting Abstract

Suche in Medline nach

  • corresponding author J. Weißmann - Department of Ophthalmology, University-Hospital Hamburg
  • S. Ehmer - Department of Ophthalmology, University-Hospital Hamburg
  • O. Strauß - Department of Ophthalmology, University-Hospital Hamburg

Evidenzbasierte Medizin - Anspruch und Wirklichkeit. 102. Jahrestagung der Deutschen Ophthalmologischen Gesellschaft. Berlin, 23.-26.09.2004. Düsseldorf, Köln: German Medical Science; 2004. Doc04dogP 177

Die elektronische Version dieses Artikels ist vollständig und ist verfügbar unter: http://www.egms.de/de/meetings/dog2004/04dog668.shtml

Veröffentlicht: 22. September 2004

© 2004 Weißmann et al.
Dieser Artikel ist ein Open Access-Artikel und steht unter den Creative Commons Lizenzbedingungen (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.de). Er darf vervielf&aauml;ltigt, verbreitet und &oauml;ffentlich zug&aauml;nglich gemacht werden, vorausgesetzt dass Autor und Quelle genannt werden.


Gliederung

Text

Objective

The Effect of VEGF-A on endothelial cells in the development of CNV is well described. RPE-cells are a main source for VEGF-A and express the receptors VEGFR-1 and VEGFR-2 as well. It has been reported that cells from excised CNV-Membranes show a higher expression of VEGFR-2. Purpose of this study is the analysis of possible autocrine "feed-back-loops" on the VEGF production by an increased secretion of VEGF from RPE-cells. These feed-back-loops may act as inducers of CNV.

Methods

VEGF-secretion was measured by the increase of concentration of VEGF in culture medium from 105 ARPE 19 cells. Concentration of VEGF was measured using a Sandwich ELISA.

Results

After the last exchange of culture medium the concentration of VEGF increased continuously and reached a maximum of 295pg/ml after 8 hours. By adding the VEGF analogon VEGF-C this basal secretion could be elevated up to 127,5% of control. After 8 hours under these conditions the VEGF concentration reached 375pg/ml.

Conclusions

VEGF-C which has a high affinity to VEGF-R2 stimulates the secretion of VEGF-A in RPE-cells efficiently. Thus, the VEGF-C dependend control of the VEGF-A secretion of the RPE is another possible mechanism causing induction of CNV in AMD.