gms | German Medical Science

102. Jahrestagung der DOG

Deutsche Ophthalmologische Gesellschaft e. V.

23. bis 26.09.2004, Berlin

Degradation of the retinal pigment epithelium by oxidative stress: protective effects of Pyruvate

Meeting Abstract

  • corresponding author O. Zeitz - Klinik und Poliklinik für Augenheilkunde, Universitätsklinikum Hamburg-Eppendorf
  • L. Schlichting - Klinik und Poliklinik für Augenheilkunde, Universitätsklinikum Hamburg-Eppendorf
  • G. Richard - Klinik und Poliklinik für Augenheilkunde, Universitätsklinikum Hamburg-Eppendorf
  • O. Strauß - Klinik und Poliklinik für Augenheilkunde, Universitätsklinikum Hamburg-Eppendorf

Evidenzbasierte Medizin - Anspruch und Wirklichkeit. 102. Jahrestagung der Deutschen Ophthalmologischen Gesellschaft. Berlin, 23.-26.09.2004. Düsseldorf, Köln: German Medical Science; 2004. Doc04dogP 129

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Veröffentlicht: 22. September 2004

© 2004 Zeitz et al.
Dieser Artikel ist ein Open Access-Artikel und steht unter den Creative Commons Lizenzbedingungen (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.de). Er darf vervielf&aauml;ltigt, verbreitet und &oauml;ffentlich zug&aauml;nglich gemacht werden, vorausgesetzt dass Autor und Quelle genannt werden.


Gliederung

Text

Objective

Reactive oxygen species (ROS) play an important role in the development of age-related macular degeneration (AMD) thus causing an intensive search for suitable radical scavengers. Pyruvate is a naturally occurring metabolite in glycolosis and its chemical structure promises an interesting high anti-oxidative potential. This work compares the anti-oxidative properties of pyruvate and vitamine C.

Methods

Initially the in-vitro anti-oxidative properties of pyruvate and vitamine C were examined without cells using the DHR-Assay for Hydroxyl radicals (MoBiTec, Germany). Subsequently ARPE-19 cells were used for the in-vivo tests of the radical scavenging potentials of vitamin C and pyruvate. The cells were cultured in FCS free RPMI medium without pyruvate and vitamine C. Afterwards they were incubated with radicals for 2 minutes using constantly circulating medium. The Hydroxyl radicals were generated through the Fenton reaction from H2O2 and Fe3+. The cell survival rate was determined with the life-dead-assay from MoBiTec, Germany.

Results

Without the addition of the scavengers the DHR-Assay exhibited an increase in average fluorescence to 8.8±0.1 (n=4) relative Light-units (LU). Vitamin C was able to reduce this increase in a concentration dependant manner to 1.0±0.0 LU at 1 mM vitamine C (n=4), pyruvate to 1.2±0.1 LU at 3 mM (n=4). In the in-vivo RPE-cell-system a concentration of only 0.01 mM pyruvate was sufficient to increase the survival rate of the ARPE-19 cells up to Sham-control levels while 10 mM vitamine C was necessary to achieve the same result.

Conclusions

In cultured ARPE-19 cells pyruvate exhibits a high anti-oxidative potential seemingly superior to that of vitamine C. Pyruvate naturally occurs in the human body and is thus presumed highly tolerable. Therefore it could be an interesting option for future use in the anti-oxidative therapy.