gms | German Medical Science

27. Deutscher Krebskongress

Deutsche Krebsgesellschaft e. V.

22. - 26.03.2006, Berlin

Disease processes may be reflected by correlations among tissue kallikrein proteases but not with proteolytic factors uPA and PAI-1 in primary ovarian carcinoma.

Meeting Abstract

  • corresponding author presenting/speaker Julia Dorn - Clinical Research Unit, Department of Obstetrics and Gynecology, Technical University of Munich, Munich, Germany, München, Deutschland
  • Nadia Harbeck - Clinical Research Unit, Department of Obstetrics and Gynecology, Technical University of Munich, Munich, Germany
  • Ronald Kates - Clinical Research Unit, Department of Obstetrics and Gynecology, Technical University of Munich, Munich, Germany
  • Viktor Magdolen - Clinical Research Unit, Department of Obstetrics and Gynecology, Technical University of Munich, Munich, Germany
  • Linda Grass - Department of Pathology and Laboratory Medicine, Mount Sinai Hospital, Toronto, Ontario, M5G 1X5, Canada
  • Antoninus Soosaipillai - Department of Pathology and Laboratory Medicine, Mount Sinai Hospital, Toronto, Ontario, M5G 1X5, Canada
  • Barbara Schmalfeldt - Clinical Research Unit, Department of Obstetrics and Gynecology, Technical University of Munich, Munich, Germany
  • Eleftherios P. Diamandis - Department of Pathology and Laboratory Medicine, Mount Sinai Hospital, Toronto, Ontario, M5G 1X5, Canada
  • Manfred Schmitt - Clinical Research Unit, Department of Obstetrics and Gynecology, Technical University of Munich, Munich, Germany

27. Deutscher Krebskongress. Berlin, 22.-26.03.2006. Düsseldorf, Köln: German Medical Science; 2006. DocOP346

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Veröffentlicht: 20. März 2006

© 2006 Dorn et al.
Dieser Artikel ist ein Open Access-Artikel und steht unter den Creative Commons Lizenzbedingungen (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.de). Er darf vervielf&aauml;ltigt, verbreitet und &oauml;ffentlich zug&aauml;nglich gemacht werden, vorausgesetzt dass Autor und Quelle genannt werden.


Gliederung

Text

Purpose: In epithelial ovarian cancer, the high mortality rate is usually ascribed to late diagnosis since epithelial ovarian tumors commonly lack early warning symptoms. Tumor-associated biomarkers, useful for diagnosis, prognosis, and/or therapy response prediction are in short supply. The serine protease urokinase-type plasminogen activator and its inhibitor PAI-1, both members of the plasminogen activation system of fibrinoylsis, and the recently described members of the interfacing system of tissue kallikrein serine proteases (hK1 to hK15) were reported to be associated with malignancy and tumor progression of early and/or advanced stage ovarian cancer patients.

Materials and methods: We determined simultaneously by ELISA the protein content of 9 proteolytic factors (uPA, PAI-1, and tissue kallikreins hK5, 6 ,7, 8, 10, 11, 13) in detergent-released extracts of 142 ovarian cancer patient primary tumor tissue specimens and conducted a direct comparison of protein expression levels of these tumor tissue-associated factors which has not been performed so far. The numbers obtained were weighted statistically and analyzed according to protein expression levels, FIGO stage, and nuclear grading.

Results: There was no difference in expression levels of any of the factors examined regarding to FIGO stage, except for hK5 which was expressed at a higher level in tumor tissues of FIGO III/IV rather than in FIGO I/II patients. hK5 was also more expressed in undifferentiated G3 tumors than in G1/2 classified ovarian cancer patient tumor specimens with higher level of cellular differentiation. We observed that hK5, 6, 7, 8, 10, and 11 were correlated with each other at a statistically significant level and that the highest level of statistically significant correlation was attributed to low-grade (G1) ovarian cancer patients. On the other hand, uPA, PAI-1, and hK13, respectively, were correlated to a few of the other proteolytic factors only, and if so, at a low level of association.

Conclusion: These data further support the notion that several of the tandemly colocalized genes of the tissue kallikrein family on locus 19q13.4 are co-expressed in ovarian cancer patients, substantiating the possible existence of a steroid hormone-driven tissue kallikrein cascade in patients with malignant disease.