Artikel
Effects of s100-B on tumor-induced brain edema in the mouse model – preliminary results
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Autoren
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Marc Schwarz
- Abteilung für Neuroradiologie, Universitätsklinikum Erlangen, Deutschland
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Arnd Dörfler
- Abteilung für Neuroradiologie, Universitätsklinikum Erlangen, Deutschland
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Tobias Engelhorn
- Abteilung für Neuroradiologie, Universitätsklinikum Erlangen, Deutschland
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Michael Buchfelder
- Neurochirurgie, Universitätsklinikum Erlangen, Deutschland
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Andrea Kleindienst
- Neurochirurgie, Universitätsklinikum Erlangen, Deutschland
| Veröffentlicht: | 16. September 2010 |
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Gliederung
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Objective: Aim of this study was to analyze the potential effects of s100-B, a small calcium-binding protein, on artificially induced brain tumors and corresponding edema in vivo as well as the time of survival of glioma-carrying laboratory mice.
Methods: For all experiments, 6x104 GFP-marked GL-261 glioma cells were implanted into the right frontal lobe of C57/bl6 s100-B over expressing and wild type mice. MRi examinations were performed in a clinical 3 Tesla MR system (TRIO, Siemens, Erlangen, Germany) 15 days after implantation using T2- and double-dose contrast-enhanced (Gadobutrol, Bayer vital, Leverkusen, Germany) T1-weighted sequences to evaluate edema- and tumor-volume in vivo.
Results: MRi examinations indicated that the tumor volumes of both groups were alike 15 days after implantation (control: 0.33±0.14 mm3; s100-B: 0.29±0.08 mm3; P=0.6). Edema volumes, on the other hand, showed different values (control: 0.44±0.19 mm3; s100-B: 0.34±0.11 mm3; P<0.05) (Abb. 1). This difference is even more striking by looking at the percentage of the particular tumor- (control: 73.2±9.9%; s100-B: 86.27±7.1%; P<0.05) and edema-volumes (control: 26.8±9.9%; s100-B: 13.7±7.1%; P<0.05) (Abb. 2). Survival studies showed unequal times of survival in both groups, whereas s100-B over expressing mice lived approximately 3 days longer compared to controls (control: 24±4 days, s100-B: 27±2.4 days; P<0.05) (Abb. 3).
Conclusions: These preliminary results indicate an influence of s100-B on the development of tumor-induced brain edemas, while tumor growth is not affected by s100-B over expression. In summary s100-B may have the potential to reduce the volume of tumor-induced brain edemas.
