gms | German Medical Science

61st Annual Meeting of the German Society of Neurosurgery (DGNC) as part of the Neurowoche 2010
Joint Meeting with the Brazilian Society of Neurosurgery on the 20 September 2010

German Society of Neurosurgery (DGNC)

21 - 25 September 2010, Mannheim

Effects of s100-B on tumor-induced brain edema in the mouse model – preliminary results

Meeting Abstract

  • Marc Schwarz - Abteilung für Neuroradiologie, Universitätsklinikum Erlangen, Deutschland
  • Arnd Dörfler - Abteilung für Neuroradiologie, Universitätsklinikum Erlangen, Deutschland
  • Tobias Engelhorn - Abteilung für Neuroradiologie, Universitätsklinikum Erlangen, Deutschland
  • Michael Buchfelder - Neurochirurgie, Universitätsklinikum Erlangen, Deutschland
  • Andrea Kleindienst - Neurochirurgie, Universitätsklinikum Erlangen, Deutschland

Deutsche Gesellschaft für Neurochirurgie. 61. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie (DGNC) im Rahmen der Neurowoche 2010. Mannheim, 21.-25.09.2010. Düsseldorf: German Medical Science GMS Publishing House; 2010. DocP1712

DOI: 10.3205/10dgnc183, URN: urn:nbn:de:0183-10dgnc1837

Published: September 16, 2010

© 2010 Schwarz et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.en). You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.


Outline

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Objective: Aim of this study was to analyze the potential effects of s100-B, a small calcium-binding protein, on artificially induced brain tumors and corresponding edema in vivo as well as the time of survival of glioma-carrying laboratory mice.

Methods: For all experiments, 6x104 GFP-marked GL-261 glioma cells were implanted into the right frontal lobe of C57/bl6 s100-B over expressing and wild type mice. MRi examinations were performed in a clinical 3 Tesla MR system (TRIO, Siemens, Erlangen, Germany) 15 days after implantation using T2- and double-dose contrast-enhanced (Gadobutrol, Bayer vital, Leverkusen, Germany) T1-weighted sequences to evaluate edema- and tumor-volume in vivo.

Results: MRi examinations indicated that the tumor volumes of both groups were alike 15 days after implantation (control: 0.33±0.14 mm3; s100-B: 0.29±0.08 mm3; P=0.6). Edema volumes, on the other hand, showed different values (control: 0.44±0.19 mm3; s100-B: 0.34±0.11 mm3; P<0.05) (Abb. 1). This difference is even more striking by looking at the percentage of the particular tumor- (control: 73.2±9.9%; s100-B: 86.27±7.1%; P<0.05) and edema-volumes (control: 26.8±9.9%; s100-B: 13.7±7.1%; P<0.05) (Abb. 2). Survival studies showed unequal times of survival in both groups, whereas s100-B over expressing mice lived approximately 3 days longer compared to controls (control: 24±4 days, s100-B: 27±2.4 days; P<0.05) (Abb. 3).

Conclusions: These preliminary results indicate an influence of s100-B on the development of tumor-induced brain edemas, while tumor growth is not affected by s100-B over expression. In summary s100-B may have the potential to reduce the volume of tumor-induced brain edemas.