gms | German Medical Science

128. Kongress der Deutschen Gesellschaft für Chirurgie

Deutsche Gesellschaft für Chirurgie

03.05. - 06.05.2011, München

Harvesting stem cell fractions from human fat tissue for therapeutic use

Meeting Abstract

  • Timo Spanholtz - Universität Witten/Herdecke, Campus Köln-Merheim, Plastische und Rekonstruktive Chirurgie, Köln
  • Oliver Thamm - Universität Witten/Herdecke, Campus Köln-Merheim, Plastische und Rekonstruktive Chirurgie, Köln
  • Nicola Bader - Universität Witten/Herdecke, Campus Köln-Merheim, Plastische und Rekonstruktive Chirurgie, Köln
  • Edmund A.M. Neugebauer - Universität Witten/Herdecke, Campus Köln-Merheim, Plastische und Rekonstruktive Chirurgie, Köln
  • Gerald Spilker - Universität Witten/Herdecke, Campus Köln-Merheim, Plastische und Rekonstruktive Chirurgie, Köln

Deutsche Gesellschaft für Chirurgie. 128. Kongress der Deutschen Gesellschaft für Chirurgie. München, 03.-06.05.2011. Düsseldorf: German Medical Science GMS Publishing House; 2011. Doc11dgch381

DOI: 10.3205/11dgch381, URN: urn:nbn:de:0183-11dgch3811

Veröffentlicht: 20. Mai 2011

© 2011 Spanholtz et al.
Dieser Artikel ist ein Open Access-Artikel und steht unter den Creative Commons Lizenzbedingungen (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.de). Er darf vervielfältigt, verbreitet und öffentlich zugänglich gemacht werden, vorausgesetzt dass Autor und Quelle genannt werden.


Gliederung

Text

Introduction: Human fat tissue is a rich source of cellular material for lipofilling and mesenchymal stem cell culture, so called adipose-derived stem cells (ASC). Basic characteristics of ASCs experience constant changes in literature. As a result, methods of isolation an primary culture as well as results of studies are often not comparable.

Materials and methods: Our group testes different protocols for isolation of ASC from liposuction tissue and solid fat. We performed (i) cell counting in primary culture, (ii) immunocytochemestry and (iii) differentiation of ASC into osteoblasts and adipocytes. Furthermore we tested different protocols for crypreservation. Our results were compared to results from recent literature. Aim was to develop an currently “optimal handling protocol” for the isolation of ASC based on available data.

Results: Various handling protocols for ASCs were found in literature. In comparison with respect to methods of isolation and primary culture the number of viable cells directly depends on the donor, the technique of harvesting and processing. In lipoaspirated fat we found about 30% of all cells damaged, while 7% of all cells were damaged when isolated from solid fat. From centrifuged stroma vascular fraction (SVF) of 1g fat tissue we harvested 6.5x105 ASCs (lipoaspirated fat) and 1x106 ASC (solid fat). We positively confirmed stem cell character by immunocytochemestry and differentiation into adipocytes and osteoblasts.

Conclusion: We are able to present basic recommendation for harvesting and culturing ASCs. Success depends on the donor, the techniques of harvesting and processing. Both parameters directly influence the quantity and quality of harvested ASCs. In literature, however, there is only little consensus about basic characteristics of ASC.