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International Conference on SARS - one year after the (first) outbreak

08. - 11.05.2004, Lübeck

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Analysis on murine-like animals carrying SARS coronavirus in the hygienic units related to SARS

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  • corresponding author presenting/speaker Jian-rong Yi - Center for Diseases Control and Prevention of Guangdong province, Guangzhou, China
  • Li-feng Lin - Center for Diseases Control and Prevention of Guangdong province, Guangzhou, China
  • Jin-hua Duan - Center for Diseases Control and Prevention of Guangdong province, Guangzhou, China

International Conference on SARS - one year after the (first) outbreak. Lübeck, 08.-11.05.2004. Düsseldorf, Köln: German Medical Science; 2004. Doc04sarsP5.01

The electronic version of this article is the complete one and can be found online at: http://www.egms.de/en/meetings/sars2004/04sars113.shtml

Published: May 26, 2004

© 2004 Yi et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.en). You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.

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Objective: To investigate SARS virus carried by murine-like animals in the hygienic units to provide evidence for looking for the origin and the infectious ways of SARS virus.

Method: Rats were caught in usual ways. Nested RT-PCR.Fluorescent quantitative PCR .Sequence analysis and Virus isolation were applied.

Result: There are 59 murine-like animals of three species caught in the hygienic units related to SARS in GuangZhou. 32 samples of anus swabs test were tested by nested RT-PCR using a pair primers recommended by WHO. The results showed that four samples were positive [Tab. 1] .The positive rate was 12.5 percent. The sequence of positive parts had 90%-96% homogeneity with SARS-CoV . The result of the cell cultivation on positive samples of PCR amplification parts was negative [Tab. 1].

Conclusion: It would be studied further whether murine-like animals can carry and transmit SARS coronavirus.