Article
Analysis on murine-like animals carrying SARS coronavirus in the hygienic units related to SARS
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Published: | May 26, 2004 |
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Objective: To investigate SARS virus carried by murine-like animals in the hygienic units to provide evidence for looking for the origin and the infectious ways of SARS virus.
Method: Rats were caught in usual ways. Nested RT-PCR.Fluorescent quantitative PCR .Sequence analysis and Virus isolation were applied.
Result: There are 59 murine-like animals of three species caught in the hygienic units related to SARS in GuangZhou. 32 samples of anus swabs test were tested by nested RT-PCR using a pair primers recommended by WHO. The results showed that four samples were positive [Tab. 1] .The positive rate was 12.5 percent. The sequence of positive parts had 90%-96% homogeneity with SARS-CoV . The result of the cell cultivation on positive samples of PCR amplification parts was negative [Tab. 1].
Conclusion: It would be studied further whether murine-like animals can carry and transmit SARS coronavirus.