gms | German Medical Science

27. Wissenschaftlicher Kongress der Deutschen Hochdruckliga

Deutsche Liga zur Bekämpfung des hohen Blutdrucks – Deutsche Hypertonie Gesellschaft e. V.

26. bis 29.11.2003, Bonn

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Adipose Tissue Gene Expression Profiling in Obesity and Hypertension

Genexpressionsprofile im Fettgewebe des Menschen bei Adipositas und Hypertonie

Meeting Abstract (Hypertonie 2003)

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  • presenting/speaker S. Engeli
  • S. Baranov
  • A. Soldatov
  • H. Lehrach
  • F.C. Luft
  • A.M. Sharma

Hypertonie 2003. 27. Wissenschaftlicher Kongress der Deutschen Hochdruckliga. Bonn, 26.-29.11.2003. Düsseldorf, Köln: German Medical Science; 2004. Doc03hochV45

The electronic version of this article is the complete one and can be found online at: http://www.egms.de/en/meetings/hoch2003/03hoch045.shtml

Published: November 11, 2004

© 2004 Engeli et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.en). You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.

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Despite increasing knowledge on the endocrine and paracrine role of adipocytes, the role of adipose tissue in obesity-related hypertension has not been investigated in a systematic manner. We currently study differential regulation of adipose-tissue genes in 80 lean and obese normotensive and hypertensive postmenopausal women in a cross-sectional study and during dietary weight reduction. All volunteers were extensively characterized by anthropometric measurements, clinical chemistry and 24h ambulatory blood pressure measurements. Total RNA was isolated from adipose tissue obtained by subcutaneous abdominal needle biopsy. Gene expression was analyzed by cDNA macroarrays and TaqMan-RT-PCR for candidate genes. The macroarrays contain about 3,000 cDNA clones selected in preliminary experiments due to differential expression between either of the lean or obese normotensive or hypertensive groups. Results on adiponectin, and 11ß-HSD1 and 2 suggest that transcriptional mechanisms lead to low adiponectin plasma levels and increased local formation of cortisol in obese subjects. Several genes involved in fatty acid synthesis and oxidation are down-regulated in obese subjects (ACACA, ECHS1, HADHSC, FACL2). The gene encoding ANP is upregulated in obese subjects. As ANP is a strong stimulator of lipolysis, this may partly contribute to high free fatty acid levels in obese subjects. Confirming previous results, the ANP-clearance receptor is upregulated in obese subjects which may explain lower ANP plasma levels especially in obese hypertensive subjects.