Article
Gene expression of components of the fibrinolytic system in serum-free cultured human keratocytes
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Published: | September 22, 2004 |
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Outline
Text
Objective
To determine the gene expression encoding for important components of the fibrinolytic system in serum-free cultured human keratocytes.
Methods
Total mRNA of confluent primary cultures of human keratocytes was isolated after 48 hours of serum-free cultivation. For each component of the fibrinolytic system reverse transcribed mRNA was measured by real time polymerase chain reaction (TaqMan® PCR) with primers and probes deduced from the human genes.
Results
In cultured human keratocytes we detected mRNA expression of plasminogen activator inhibitor-1 (PAI-1), thrombin activatable fibrinolysis inhibitor (TAFI), tissue-type plasminogen activator (tPA), urokinase-type plasminogen activator (uPA), protein C and protein S.
Conclusions
We localized the gene expression of important components of the fibrinolytic system in cultured keratocytes of the human eye. PAI-1 and TAFI are main regulators of the fibrinolytic system. Both inhibitors reduce plasminogen activity and attenuate fibrinolysis and proteolysis. In addition, PAI-1 leads to decreased dissolution of extracellur matrix. Therefore, stimulation of PAI-1 and TAFI production may be involved in inflammation and wound healing processes of the cornea.
Supported by Deutsche Forschungsgemeinschaft (DFG), ER 259/1