gms | German Medical Science

102. Jahrestagung der DOG

Deutsche Ophthalmologische Gesellschaft e. V.

23. bis 26.09.2004, Berlin

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Gene expression of components of the fibrinolytic system in serum-free cultured human keratocytes

Meeting Abstract

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  • corresponding author M. W. Meyer - Augenklinik der Medizinischen Hochschule Hannover, Hannover
  • M. Depka - Hämatologie der Medizinischen Hochschule Hannover, Hannover
  • A. Meyer - Augenklinik der Medizinischen Hochschule Hannover, Hannover
  • A. Schröder - Augenklinik der Medizinischen Hochschule Hannover, Hannover
  • R. Winter - Augenklinik der Medizinischen Hochschule Hannover, Hannover
  • C. Erb - Augenklinik der Medizinischen Hochschule Hannover, Hannover

Evidenzbasierte Medizin - Anspruch und Wirklichkeit. 102. Jahrestagung der Deutschen Ophthalmologischen Gesellschaft. Berlin, 23.-26.09.2004. Düsseldorf, Köln: German Medical Science; 2004. Doc04dogDO.08.11

The electronic version of this article is the complete one and can be found online at: http://www.egms.de/en/meetings/dog2004/04dog078.shtml

Published: September 22, 2004

© 2004 Meyer et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.en). You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.

Outline

Text

Objective

To determine the gene expression encoding for important components of the fibrinolytic system in serum-free cultured human keratocytes.

Methods

Total mRNA of confluent primary cultures of human keratocytes was isolated after 48 hours of serum-free cultivation. For each component of the fibrinolytic system reverse transcribed mRNA was measured by real time polymerase chain reaction (TaqMan® PCR) with primers and probes deduced from the human genes.

Results

In cultured human keratocytes we detected mRNA expression of plasminogen activator inhibitor-1 (PAI-1), thrombin activatable fibrinolysis inhibitor (TAFI), tissue-type plasminogen activator (tPA), urokinase-type plasminogen activator (uPA), protein C and protein S.

Conclusions

We localized the gene expression of important components of the fibrinolytic system in cultured keratocytes of the human eye. PAI-1 and TAFI are main regulators of the fibrinolytic system. Both inhibitors reduce plasminogen activity and attenuate fibrinolysis and proteolysis. In addition, PAI-1 leads to decreased dissolution of extracellur matrix. Therefore, stimulation of PAI-1 and TAFI production may be involved in inflammation and wound healing processes of the cornea.

Supported by Deutsche Forschungsgemeinschaft (DFG), ER 259/1