Article
Multiparametric miniaturized immunoassays to characterize biopsy-derived breast tumor tissue
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Published: | March 20, 2006 |
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Miniaturized and parallelized sandwich immunoassays are of general interest for all proteomic and diagnostic approaches in which several parameters have to be determined from small samples, e.g. biopsy material. Appropriate sensitivity, reproducibility, and robustness have to be demonstrated before such protein microarray technology can be used to characterize clinical samples and generate reliable data sets. Parallel to establishing a number of reliable assays to measure conventional prognostic and predictive factors as well as potential drug targets and promising candidate proteins, we collected 120 large core needle breast biopsies taken under 3D-ultrasound guidance to validate this diagnostic approach. From these samples (mean weight of 20 mg) 300-1000 µg protein was solubilized allowing the simultaneous quantification of more than hundred proteins. We provide an overall analysis of the initially generated data set consisting of >40 analyte concentrations per biopsy to evaluate the feasibility of this diagnostic approach. The concordance with conventional methods is shown by comparison with routine procedures to assess tumor cells’ expressions of the estrogen receptor and Her-2.