gms | German Medical Science

27th German Cancer Congress Berlin 2006

German Cancer Society (Frankfurt/M.)

22. - 26.03.2006, Berlin

Multiparametric miniaturized immunoassays to characterize biopsy-derived breast tumor tissue

Meeting Abstract

  • corresponding author presenting/speaker Helmut Deißler - Universitätsfrauenklinik Ulm, Deutschland
  • Georg Sauer - Universitätsfrauenklinik Ulm
  • Nicole Schneiderhan-Marra - Naturwissenschaftliches und Medizinisches Institut an der Universität Tübingen, Reutlingen
  • Martin Bäuerle - Universitätsfrauenklinik Ulm
  • Rolf Kreienberg - Universitätsfrauenklinik Ulm
  • Thomas Joos - Naturwissenschaftliches und Medizinisches Institut an der Universität Tübingen, Reutlingen
  • Helmut Deissler - Universitätsfrauenklinik Ulm

27. Deutscher Krebskongress. Berlin, 22.-26.03.2006. Düsseldorf, Köln: German Medical Science; 2006. DocPO425

The electronic version of this article is the complete one and can be found online at:

Published: March 20, 2006

© 2006 Deißler et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.



Miniaturized and parallelized sandwich immunoassays are of general interest for all proteomic and diagnostic approaches in which several parameters have to be determined from small samples, e.g. biopsy material. Appropriate sensitivity, reproducibility, and robustness have to be demonstrated before such protein microarray technology can be used to characterize clinical samples and generate reliable data sets. Parallel to establishing a number of reliable assays to measure conventional prognostic and predictive factors as well as potential drug targets and promising candidate proteins, we collected 120 large core needle breast biopsies taken under 3D-ultrasound guidance to validate this diagnostic approach. From these samples (mean weight of 20 mg) 300-1000 µg protein was solubilized allowing the simultaneous quantification of more than hundred proteins. We provide an overall analysis of the initially generated data set consisting of >40 analyte concentrations per biopsy to evaluate the feasibility of this diagnostic approach. The concordance with conventional methods is shown by comparison with routine procedures to assess tumor cells’ expressions of the estrogen receptor and Her-2.