gms | German Medical Science

56. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie e. V. (DGNC)
3èmes journées françaises de Neurochirurgie (SFNC)

Deutsche Gesellschaft für Neurochirurgie e. V.
Société Française de Neurochirurgie

07. bis 11.05.2005, Strasbourg

Article

Send article

Molecular neuropathology of epilepsy-associated glioneuronal tumors: functional genome analysis in gangliogliomas

Molekulare Neuropathologie Epilepsie-assoziierter glioneuronaler Tumore: funktionelle Genomanalyse in Gangliogliomen

Meeting Abstract

Search Medline for

  • corresponding author J. Fassunke - Dept. of Neuropathology, University of Bonn Medical Center, Germany
  • V. Schick - Dept. of Neuropathology, University of Bonn Medical Center, Germany
  • A. Tresch - Fraunhofer Society, SCAI, St. Augustin, Germany
  • P. Koch - Dept. of Reconstructive Neurobiology, University of Bonn Medical Center, Germany
  • C. Ullmann - Dept. of Neuropathology, University of Bonn Medical Center, Germany
  • C. E. Elger - Dept. of Epileptology, University of Bonn Medical Center, Germany
  • J. Schramm - Dept. of Neurosurgery, University of Bonn Medical Center, Bonn
  • A. J. Becker - Dept. of Neuropathology, University of Bonn Medical Center, Germany

Deutsche Gesellschaft für Neurochirurgie. Société Française de Neurochirurgie. 56. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie e.V. (DGNC), 3èmes journées françaises de Neurochirurgie (SFNC). Strasbourg, 07.-11.05.2005. Düsseldorf, Köln: German Medical Science; 2005. DocP016

The electronic version of this article is the complete one and can be found online at: http://www.egms.de/en/meetings/dgnc2005/05dgnc0284.shtml

Published: May 4, 2005

© 2005 Fassunke et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.en). You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.

Outline

Text

Objective

The comprehensive analysis of disease-related gene transcripts gains increasing importance for understanding the molecular basis of human diseases. Gangliogliomas constitute the most frequent glioneuronal tumor entity occurring in patients with pharmacoresistant epilepsy. We here report first results of a microarray-based expression profile analysis in gangliogliomas.

Methods

Microarray analysis (Affymetrix U133A) was carried out comparing expression patterns in ganglioglioma vs. adjacent normal control tissue (n= 6 patients). Real time RT-PCR analysis was performed to validate differential gene expression patterns.

Results

A total number of 95 genes was found to be differentially expressed in gangliogliomas vs. controls. Stratification by gene ontology analysis revealed several differentially expressed genes related to neuronal differentiation and migration. In particular, protein kinase C, beta 1 type as well as its binding partner NELL2 are significantly reduced in expression in gangliogliomas.

Conclusions

Gene chip technologies open new avenues for the identification of candidate molecules and mechanisms involved in the pathogenesis of gangliogliomas. Current experiments focus on functional analyses of candidate genes using siRNA approaches.