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International Conference on SARS - one year after the (first) outbreak

08. - 11.05.2004, Lübeck

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Evaluation of the real-time RT-PCR for the detection of SARS-CoV

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  • corresponding author presenting/speaker Yoon-Seok Chung - Division of Respiratory Viruses, Department of Virology, National Institute of Health, Center for Disease Control and Prevention, Seoul, Korea
  • Min-Kyung Kim - Division of Respiratory Viruses, Department of Virology, National Institute of Health, Center for Disease Control and Prevention, Seoul, Korea
  • Hyang-Min Cheong - Division of Respiratory Viruses, Department of Virology, National Institute of Health, Center for Disease Control and Prevention, Seoul, Korea
  • In-Soo Kim - Division of Respiratory Viruses, Department of Virology, National Institute of Health, Center for Disease Control and Prevention, Seoul, Korea
  • Joo-Yeon Lee - Division of Respiratory Viruses, Department of Virology, National Institute of Health, Center for Disease Control and Prevention, Seoul, Korea
  • Mi-Sun Park - Division of Respiratory Viruses, Department of Virology, National Institute of Health, Center for Disease Control and Prevention, 5 NokbunDong, Eun-pyungGu, Seoul, Korea
  • Chun Kang - Division of Respiratory Viruses, Department of Virology, National Institute of Health, Center for Disease Control and Prevention, Seoul, Korea

International Conference on SARS - one year after the (first) outbreak. Lübeck, 08.-11.05.2004. Düsseldorf, Köln: German Medical Science; 2004. Doc04sarsP8.03

The electronic version of this article is the complete one and can be found online at: http://www.egms.de/en/meetings/sars2004/04sars120.shtml

Published: May 26, 2004

© 2004 Chung et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.en). You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.

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Severe acute respiratory syndrome (SARS) is caused by a novel coronavirus, called the SARS coronavirus (SARS-CoV). The genome of SARS-CoV has been sequenced and it is not related to any of the previously known human or animal coronaviruses. The virus can be found in nasopharyngeal aspirate, urine and stools of SARS patients. A real-time RT-PCR assay is able to detect SARS-CoV in nasopharyngeal aspirates of approximately 90% of patients with SARS within the first 3 days of illness. We examined the real-time RT-PCR based on multiple primer and probe sets located in different regions of the SARS-CoV genome. And we attempted to determine the optimal primer and probe sets useful for the diagnosis of the acute respiratory patients during the phase of the SARS outbreak in Korea. The real-time RT-PCR was evaluated with serial dilutions of inactivated standard RNA preparation from ENVID, Germany and SARS-CoV Urbani strain from CDC, USA. Also commercial SARS-CoV real-time RT-PCR tests were run in parallel. The result of evaluation of real-time RT-PCR confirmed that primer and probe sets located in polymerase (pol) gene and nucleocapsid (N) gene showed higher sensitivity than other regions. Taken together, it is suggested that the real-time RT-PCR for detection of SARS-CoV be implemented with 2 primer and probe sets located in pol gene and another primer and probe set in N gene of the SARS-CoV.