gms | German Medical Science

Infektiologie Update 2016: 25. Jahrestagung der Paul-Ehrlich-Gesellschaft für Chemotherapie (PEG)

Paul-Ehrlich-Gesellschaft für Chemotherapie (PEG)

06.-08.10.2016, Rostock

Article

Send article

Prevalence, antimicrobial resistance and exotoxin profiling of Panton-Valentine leucocidin positive Staphylococcus aureus isolated from blood culture

Meeting Abstract

Search Medline for

  • author Heimo Lagler - Medical University of Vienna, Austria, Department of Medicine 1, Division of Infectious Diseases and Tropical Medicine, Vienna, Austria
  • Victoria Burda - Medical University of Vienna, Austria, Department of Medicine 1, Division of Infectious Diseases and Tropical Medicine, Vienna, Austria
  • Markus Obermüller - Medical University of Vienna, Austria, Department of Medicine 1, Division of Infectious Diseases and Tropical Medicine, Vienna, Austria
  • author Wolfgang Graninger - Institute of Infectiology, Karl Landsteiner Society, Vienna, Austria

Infektiologie Update 2016. 25. Jahrestagung der Paul-Ehrlich-Gesellschaft für Chemotherapie (PEG). Rostock, 06.-08.10.2016. Düsseldorf: German Medical Science GMS Publishing House; 2016. Doc16peg21

doi: 10.3205/16peg21, urn:nbn:de:0183-16peg219

Published: September 30, 2016

© 2016 Lagler et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 License. See license information at http://creativecommons.org/licenses/by/4.0/.

Outline

Text

Staphylococcus aureus (SA) is a ubiquitous bacteria and one of the most frequent causes of bacterial infections. The methicillin resistant (MR) SA can cause severe infections like bacteremia (SAB) and due to its limited therapeutic options a challenge in clinical practice. Community acquired (CA) MRSA is known to have an increased level of Panton - Valentine leucocidin (PVL). PVL induced infections are mainly necrotizing skin and soft tissue infections and necrotizing pneumonia. The current prevalence, antimicrobial resistance and exotoxin profile of PVL positive SAB strains are of great interest.

Materials and Methods: We investigated a collection of 1363 SAB strains, collected from 2000 to 2015 at the Medical University of Vienna, for the presence of the PVL gene LukSF by GenoType MRSA (Hain Lifescience, Germany). The LukSF positive SAB strains were analyzed for antimicrobial resistance according to European Committee on Antimicrobial Susceptibility Testing (EUCAST) and for 30 exotoxin genes (sea-see, tst-1, seg-ser, seu, eta, etb, ahl, bhl, ghlabc, ghlvar, dhl, lukDE, lukM) by polymerase chain reaction (PCR).

Results: The PVL gene lukSF was found on an average of 1.1% (0% to 3%) of the SAB isolates per year. In all PVL positive strains the exotoxin genes for haemolysin alpha (hla) and –delta (hld) were detected most frequently (in 86,6% and 100%), however sec, see, tst-1, sel and lukM couldn’t be detected in any of the samples. The highest number of resistance rate was found against penicillin G in 13 of 15 samples, only 2 of 15 were MRSA. There was no resistance found against fosfomycin, fusidic acid, linezolid, vancomycin, teicoplanin and mupirocin.

Conclusion: The observation of a 16 year period showed no increase in the constant low number of PVL positive SAB strains. Furthermore PVL positive CA-MRSA strains were rarely found in blood cultures. There is no significant correlation between the number of exotoxin genes and antibiotic resistance.

The study was funded by the PEG-Stiftung.