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14th Triennial Congress of the International Federation of Societies for Surgery of the Hand (IFSSH), 11th Triennial Congress of the International Federation of Societies for Hand Therapy (IFSHT)

17.06. - 21.06.2019, Berlin

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Vancomycin is superior to Plectasin against Staphyloccus aureus periprosthetic osteomyelitis in rats

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  • presenting/speaker Niels Henrik Søe - Handsection, Department of Ortopaedic, Hellerup, Denmark
  • Asger Lundorff Jensen - Institut for Veterinær-og Husdyrvidenskab, Frederiksberg, Denmark
  • Britt Siesing-Mejer - Department of Orthopaedic, Hillerød University Hospital, Hillerød, Denmark
  • Steen Seier Poulsen - Endocrinology and Metabolism, Panum Instituttet, Copenhagen, Denmark
  • Janne Koch - Leo Pharma, Ballerup, Denmark
  • Helle Krogh Johansen - Mikrobiologisk afd., Copenhagen, Denmark

International Federation of Societies for Surgery of the Hand. International Federation of Societies for Hand Therapy. 14th Triennial Congress of the International Federation of Societies for Surgery of the Hand (IFSSH), 11th Triennial Congress of the International Federation of Societies for Hand Therapy (IFSHT), 11th Triennial Congress of the International Federation of Societies for Hand Therapy (IFSHT). Berlin, 17.-21.06.2019. Düsseldorf: German Medical Science GMS Publishing House; 2020. DocIFSSH19-554

doi: 10.3205/19ifssh0103, urn:nbn:de:0183-19ifssh01032

Published: February 6, 2020

© 2020 Søe et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 License. See license information at http://creativecommons.org/licenses/by/4.0/.

Outline

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Objectives/Interrogation: Commonly used antibiotics cannot always control S. aureus associated infections in orthopaedic and hand implants.

We investigated the ability of vancomycin and plectasin to eradicate S. aureus in a knee prosthesis model of osteomyelitis in rats. We compared Plectasin in a 20 and 40 mg concentration to vancomycin 20 mg, all antibiotics were given i.p. daily. Plectasin is a peptide antibiotic with therapeutic potential from a saprophytic fungus.

Methods: Thirty Sprague-Dawley rats had prosthesis inserted and divided into three groups (N=10). Vancomycin group including 2 controls and two Plectasin groups (N= 10) 20 mg and (N= 10) 40 mg including 2 controls in each groups. All groups were infected with S. aureus MN8, ica+103 in the tibia and the femur marrow before insertion of the prosthesis. Control rats were given NaCl i.p. After two weeks, the rats were sacrificed and all specimens were analysed.

Results: One rat in the 20 mg Plectasin group and four in the 40 mg Plectasin group died of anaphylactic shock (histamine release). The 20 and 40 mg Plectasin groups both showed a decrease of bacteria but it was not as efficiently eradicated as in the vancomycin group.

Conclusions: Plectasin treatment against S. aureus osteomyelitis reduced the infection. However, Plectasin released histaminestrongly after one day and some of the rats died especially in the 40 mg group. In contrast, Vancomycin reduced the infection significantly in almost of the parameters