gms | German Medical Science

28. Wissenschaftlicher Kongress der Deutschen Hochdruckliga

24. bis 27.11.2004, Hannover

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Identification of endogenous diadenosine tetraphosphate, diadenosine pentaphosphate and diadenosine hexaphosphate in human myocardial tissue

Identifizierung des Diadenosintetraphosphats, Diadenosinpentaphosphats und Diadenosinhexaphosphats als neue Hormone des menschlichen Herzgewebes

Meeting Abstract (Hypertonie 2004)

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  • V. Jankowski - Charité - Universitätmedizin (Berlin, D)
  • W. Zidek - Charité - Universitätmedizin (Berlin, D)
  • J. Jankowski - Charité - Universitätmedizin (Berlin, D)

Hypertonie 2004. 28. Wissenschaftlicher Kongress der Deutschen Hochdruckliga. Hannover, 24.-27.11.2004. Düsseldorf, Köln: German Medical Science; 2005. Doc04hochP114

The electronic version of this article is the complete one and can be found online at: http://www.egms.de/en/meetings/hoch2004/04hoch114.shtml

Published: August 10, 2005

© 2005 Jankowski et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.en). You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.

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Diadenosine polyphosphates have been characterised as extracellular mediators controlling numerous physiological effects. In this study diadenosine tetraphosphate (Ap4A), diadenosine pentaphosphate (Ap5A) and diadenosine hexaphosphate (Ap6A.) were isolated and identified in human myocardial tissue. Human myocardial tissue was homogenized and fractionated by affinity-, displacement-, anion-exchange-, and reversed-phase chromatographies. In fractions purified to homogeneity, Ap4A, Ap5A and Ap6A were revealed by matrix assisted laser desorption/ionization (MALDI) mass spectrometry and UV-spectroscopy. These diadenosine polyphosphates were further identified by enzymatic analysis, which demonstrated an interconnection of the phosphate groups with the adenosines in the 5´-positions of the riboses. Furthermore, Ap4A, Ap5A and Ap6A were found in human cardiac specific granules and the amount of Ap4A, Ap5A and Ap6A was estimated in the range of about 500 µmol/L. In conclusion, Ap4A, Ap5A and Ap6A are present in human myocardial specific granules and may affect heart function in an autocrine loop after being released by stimulation.