Article
Agonistic AT1-Receptor Autoantibodies and Monocyte-Stimulation in Hypertensive Patients
Agonistische AT1-Rezeptor Autoantikörper und Monozyten-Stimulation bei Hypertensiven Patienten
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Published: | August 10, 2005 |
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Objective: Agonistic AT1-receptor autoantibodies (AT1-AA) have been described in hypertensive and preeclamptic patients. Furthermore, monocytes are activated in hypertensive patients. We investigated the ability of AT1-AA to stimulate monocytes from hypertensive and normotensive persons. The adhesiveness of the monocytes to endothelial cell layers and the tissue factor expression were determined.
Methods: Peripheral blood monocytes from 17 patients with essential hypertension and from 20 normotensive subjects were isolated by Dynabeads and used in adhesion experiments. Adherence assays and Western blotting were done.
Results: Monocyte adhesion to human aortic endothelial cell layers was significantly higher after stimulation with AT1-AA compared to no stimulation. The effect was blocked with tissue factor antibody or epitope peptide preincubation. Eposartan was partially effective in blocking the effects. Western blotting after AT1-AA or Ang II stimulation showed that the monocytes expressed tissue factor.
Conclusion: These data show that monocytes can be stimulated by AT1-AA to adhere and produce tissue factor. They underscore the importance of monocyte activation in hypertensive patients. The relevance of AT1-AA in hypertension will require further studies.