gms | German Medical Science

87th Annual Meeting of the German Society of Oto-Rhino-Laryngology, Head and Neck Surgery

German Society of Oto-Rhino-Laryngology, Head and Neck Surgery

04.05. - 07.05.2016, Düsseldorf

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The localization of sarco-endoplasmic reticulum Ca2+ ATPases (SERCAs) in the murine pharyngeal and laryngeal muscles

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  • corresponding author Mohammed Elrabie Ahmed - Kyoto Prefectural University of Medicine Japan, Kyoto, Japan
  • Bando Hideki - Kyoto prefectural University of Medicine, Kyoto, Japan
  • Hirota Ryuichi - Kyoto prefectural University of Medicine, Kyoto, Japan
  • Mohamed Ahmed - Sohag University, Faculty of Medicine, Sohag, Egypt
  • Hesham Abd el Fattah - University, Faculty of Medicine, Alexandria, Egypt

Deutsche Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie. 87. Jahresversammlung der Deutschen Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie. Düsseldorf, 04.-07.05.2016. Düsseldorf: German Medical Science GMS Publishing House; 2016. Doc16hnod001

doi: 10.3205/16hnod001, urn:nbn:de:0183-16hnod0010

Published: March 30, 2016

© 2016 Ahmed et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 License. See license information at http://creativecommons.org/licenses/by/4.0/.

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Introduction: The laryngeal and pharyngeal muscles are still relatively understudied. They stand apart from the limb skeletal muscle in several anatomical and physiological aspects.

Sarcoplasmic reticulum is a specialized Ca2+ storage membrane system that plays a vital role in the muscle contractile function by its ability to regulate intracellular Ca2+ concentration. Ca2+ release from the SR initiates muscle contraction, whereas Ca2+ reuptake lowers cytosolic calcium, causing muscle relaxation. Hence the events that regulate Ca2+ release and removal affects cytosolic calcium levels, the rate and extent of muscle contraction and relaxation. The Ca2+ reuptake function of the SR is regulated by a transmembrane Ca2+ transport pump, the sarco(endo)plasmic reticulum Ca2+ ATPase (SERCA). The SERCA pump is expressed at high levels in cardiac, skeletal, and smooth muscle cells.

Methods: Immunohistochemistry study of SERCA1, SERCA2 and myosin heavy chain type I and II for the laryngeal and pharyngeal muscles of normal adult male Wistar rat was done.

Results: The expression of each of each antibody was identified. Cell counting was made using Imagej to identify percentage of each.

Conclusions: We could identify unique pattern of expression of SERCA in rat laryngeal and hypopharyngeal muscle and correlate with the function of each muscle.

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