gms | German Medical Science

The application of tissue tolerable plasma (TTP) on the skin is of particular importance in dermatology, as effective therapies for chronic wounds are mostly insufficient. The positive effects of TTP on wound healing result from the reduction of the bacterial load in wound areas as well as from the activation of epithelial cell proliferation. The comprehension of the mechanisms of the cellular modulation may be hampered by the complex composition of plasma, which consists of charged particles, reactive oxygen species (ROS) and several types of radiation (V-UV, UVA, UVB).

The aim of the present study was to identify the effects of varying doses of TTP treatment on molecular pathways in airway epithelial cells at several time points. An in-vitro wound healing assay was applied to determine the macroscopically optimal dosage of TTP treatment for proliferation, while a 2D-difference gel-electrophoresis (2D-DIGE) approach was used to quantify the proteomic changes and to evaluate the balance of beneficial and adverse effects due to TTP application. Differentially expressed proteins and TTP-mediated post translational protein modifications have been identified by MALDI-TOF/TOF- and LC-ESI-mass spectrometry.

Results of an in-vitro wound-model highlight the dose dependent effects of TTP treatment. Treatment of cells improved the wound healing rate by nearly 15% compared to untreated cells. Additionally, analyses on protein level supported the wound healing model, as alterations in protein expression profiles increased with the applied dose and the induction of cell proliferation. However, higher doses of TTP are also a more potent cause for cellular imbalance, leading to a higher rate of cell death and apoptosis.

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