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85th Annual Meeting of the German Society of Oto-Rhino-Laryngology, Head and Neck Surgery

German Society of Oto-Rhino-Laryngology, Head and Neck Surgery

28.05. - 01.06.2014, Dortmund

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Could Confocal Laser Endomicroscopy be the missing link?

Meeting Abstract

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  • corresponding author Mihael Rudes - HNO-Klinik, Universitätsklinikum des Saarlandes, Homburg/S., DE
  • Bernhard Schick - HNO-Klinik, Universitätsklinikum des Saarlandes, Homburg/S., DE

Deutsche Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie. 85. Jahresversammlung der Deutschen Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie. Dortmund, 28.05.-01.06.2014. Düsseldorf: German Medical Science GMS Publishing House; 2014. Doc14hnod048

doi: 10.3205/14hnod048, urn:nbn:de:0183-14hnod0488

Published: April 14, 2014

© 2014 Rudes et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.en). You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.

Outline

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Introduction: Presently diagnostic gold standard in evaluation of lesions of the upper aerodigestive tract (UADT) is microscopically or endoscopically controlled biopsy followed by histological examination. Confocal Laser Endomicroscopy (CLE) is a novel ultra high magnification endoscopic method, which allows real-time histological assessment at the cellular and subcellular level. This technique offers an immediate “optical biopsies” and predicts histological diagnosis with high accuracy as demonstrated in the field of gastroenterology.

Methods: In this pilot study conducted at the University Department of Otorhinolaryngology and Head and Neck surgery, Saarland University Medical Centre, Homburg, our group investigated 50 human head and neck tissue specimens ex vivo, including normal and endoscopically altered mucosa and solid tissue. After the application of topical fluorescent agents, resected specimens were analysed with Cellvizio Probe-based CLE. Results were compared to conventional histology.

Results: CLE provided high quality microscopic images of the superficial tissue layers with remarkable resemblance to the conventional histology with high level of accuracy. Moreover, CLE allowed evaluation of the specimen sections on the vertical as well as horizontal axis, whereas histological analysis traditionally enabled evaluation only on the vertical axis.

Conclusion: The preliminary results of this study confirm potential application of the CLE in the assessment of the head and neck pathology. It promises to serve a basis for design of prospective clinical trial that should critically reveal its usefulness in the clinical setting.

Unterstützt durch: Universitätsklinikum des Saarlandes

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