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83rd Annual Meeting of the German Society of Oto-Rhino-Laryngology, Head and Neck Surgery

German Society of Oto-Rhino-Laryngology, Head and Neck Surgery

16.05. - 20.05.2012, Mainz

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Temporal onset pattern of Cre recombinase under promotor Brn3.1 in hair cells

Meeting Abstract

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  • author presenting/speaker Silke Frenz - Univ. Dept. Otorhinolaryngology, Plastic, Aesthetic and Reconstructive Head and Neck Surgery, Comprehensive Hearing Center, Wuerzburg, Germany
  • Katja John - Univ. Dept. Otorhinolaryngology, Plastic, Aesthetic and Reconstructive Head and Neck Surgery, Comprehensive Hearing Center, Wuerzburg, Germany
  • author Kristen Rak - Univ. Dept. Otorhinolaryngology, Plastic, Aesthetic and Reconstructive Head and Neck Surgery, Comprehensive Hearing Center, Wuerzburg, Germany
  • Sibylle Jablonka - Institute for Clinical Neurobiology, Wuerzburg, Germany
  • Rudolf Hagen - Univ. Dept. Otorhinolaryngology, Plastic, Aesthetic and Reconstructive Head and Neck Surgery, Comprehensive Hearing Center, Wuerzburg, Germany
  • corresponding author Robert Mlynski - Univ. Dept. Otorhinolaryngology, Plastic, Aesthetic and Reconstructive Head and Neck Surgery, Comprehensive Hearing Center, Wuerzburg, Germany

German Society of Oto-Rhino-Laryngology, Head and Neck Surgery. 83rd Annual Meeting of the German Society of Oto-Rhino-Laryngology, Head and Neck Surgery. Mainz, 16.-20.05.2012. Düsseldorf: German Medical Science GMS Publishing House; 2012. Doc12hno31

doi: 10.3205/12hno31, urn:nbn:de:0183-12hno318

Published: July 23, 2012

© 2012 Frenz et al.
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Outline

Text

Several mouse mutant strains serve as models of human hearing disorders. Unfortunately, germ line mutation of a gene that is expressed in many tissues and many cell types often results in embryonic lethality. Cre recombinase mediated tissue-specific gene targeting is a powerful tool for studying development and differentiation of inner ear cells. The aim of this study was to identify the temporal onset pattern of Cre recombinase activity under the promoter Brn3.1. Brn3.1 IRES Cre mice were cross bred with floxed lacZ and EYFP reporter mice, respectively.

The cochleae of postnatal mice were fixed; the organ of Corti was dissected and stained with antibodies against EYFP and lacZ, respectively. To ensure normal hearing background mice from all used strains and their recombinant offspring were tested using ABR audiometry and DPOAE mesasurement.

All mice showed normal ABR and DPOAE values, thereby confirming that neither insertion of the IRES Cre cassette into the Brn3.1 gene led to abnormal auditory development nor the reporter strains showed inherited hearing disorders.

P14 mice showed Cre recombinase activity in outer hair cells in a mosaic pattern. This irregular activity may be due to a cell specific onset or offset pattern. Further investigation is needed to reveal the complete timeframe for Cre recombinase activity under the Brn3.1 promotor based on these findings.