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82nd Annual Meeting of the German Society of Oto-Rhino-Laryngology, Head and Neck Surgery

German Society of Oto-Rhino-Laryngology, Head and Neck Surgery

01.06. - 05.06.2011, Freiburg

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Examination of different cell densities of ADSC for an optimal chondrogenesis in collagen type I- and agarose-hydrogels

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  • corresponding author presenting/speaker Sascha Artus - Universität Würzburg, Würzburg, Germany
  • Antje Technau - Klinik und Poliklinik für Hals-, Nasen- und Ohrenkrankheiten, plastische und ästhetische Operationen, Würzburg, Germany
  • Katrin Frölich - Klinik und Poliklinik für Hals-, Nasen- und Ohrenkrankheiten, plastische und ästhetische Operationen, Würzburg, Germany
  • Norbert Kleinsasser - Klinik und Poliklinik für Hals-, Nasen- und Ohrenkrankheiten, plastische und ästhetische Operationen, Würzburg, Germany

German Society of Oto-Rhino-Laryngology, Head and Neck Surgery. 82nd Annual Meeting of the German Society of Oto-Rhino-Laryngology, Head and Neck Surgery. Freiburg, 01.-05.06.2011. Düsseldorf: German Medical Science GMS Publishing House; 2011. Doc11hno68

doi: 10.3205/11hno68, urn:nbn:de:0183-11hno682

Published: August 3, 2011

© 2011 Artus et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.en). You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.

Outline

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Introduction: In order to develop cell-based strategies for the treatment of vocal-fold pareses by means of tissue-engineering, the chondrogenic differentiation of Adipose Tissue Derived Stem Cells (ADSC) in injectable hydrogels was examined.

Methods: ADSC of various cell concentrations were differentiated into cartilaginous cells over 14 days in agarose- (n=3) and collagen I-hydrogels (n=5) with TGF-ß3. Afterwards the concentration leading to the highest cartilage similarity was evaluated. The extent of differentiation was examined with Alcian Blue-, Safranin O- and Hematoxylin-Eosin- stain and PCR.

Results: The collagen gels showed a positive Alcian Blue stain starting from 5x104 cells/ml to 5x105 cells/ml depending on the cell-donor. The intensity of the stain varied with increasing cell numbers. The Safranin O-stain was positive at only one donor in the collagen gels while in agarose gels both stains were weakly displayed. The PCR confirmed the histologically observed dependance of differentiation on the donor and cell number.

Conclusion: A relevant chondrogenic differentiation with the cell numbers used could be observed in collagen I-hydrogels, only. An optimisation of the chondrogenic differentiation and thus the production of the extracellular matrix in injectable gels is possible by means of varying the cell density. The best cell density observed in this assay will now be transferred into an animal model to evaluate its applicability as a filling substance in vivo.