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77th Annual Meeting of the German Society of Oto-Rhino-Laryngology, Head and Neck Surgery

German Society of Oto-Rhino-Laryngology, Head and Neck Surgery

24.05. - 28.05.2006, Mannheim

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Immunohistological detection of the chemokines IL-8, MCP-1, MIP-1alpha and RANTES - Study of different chemokine antibodies

Immunhistologische Detektierung der Chemokine IL--8, MCP-1, MIP-1alpha und RANTES - Methodenstudie unter Verwendung unterschiedlicher Antikörper

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  • corresponding author presenting/speaker Ulrike Förster - ENT Department, Berlin, Germany
  • Michael Sticherling - Dermatology Department, Leipzig, Germany
  • Heidi Olze - ENT Department, Berlin, Germany

German Society of Otorhinolaryngology, Head and Neck Surgery. 77th Annual Meeting of the German Society of Otorhinolaryngology, Head and Neck Surgery. Mannheim, 24.-28.05.2006. Düsseldorf, Köln: German Medical Science; 2006. Doc06hno001

The electronic version of this article is the complete one and can be found online at: http://www.egms.de/en/meetings/hno2006/06hno001.shtml

Published: September 7, 2006

© 2006 Förster et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.en). You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.

Outline

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Introduction: Chemokines have recently been described in the pathogenesis of polyposis nasi. However, the immunohistological detection of chemokines is difficult. The aim of the study was to investigate the immunohistological examination. Following specimens of dermatoses were examinated. From in vitro examinations the investigated chemokines were described in the skin.

Methods: Biopsies of patients with psoriasis (n=10), eczema (n=10) and normal skin (n=10) were examined. Cryostat sections were stained with the ABC-kit. Twelve monoclonal and polyclonal antibodies were investigated (IL-8, MCP-1, MIP-1alpha and RANTES). Secondly the chemokine antibodies were tested in a solid phase ELISA.

Results: From 5 IL-8 antibodies one monoclonal antibodiy revealed a specific suprabasal immunoreactivity with less staining according to the grade of inflammation. Another monoclonal IL-8 antibody showed a specific homogeneous reaction in psoriasis, eczema and normal skin. One of 2 tested MIP-1alpha antibodies revealed a specific immunoreactivity in the stratum corneum of normal skin. The other antibodies were negative. The solid phase ELISA revealed predominantly specific reactions.

Conclusions: The immunohistological differences depended on the antibodies. For the characterization of chemokines immunohistological examinations are unreliable and should be complemented through other immunoassays (e.g. ELISA/ PCR).