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76th Annual Meeting of the German Society of Oto-Rhino-Laryngology, Head and Neck Surgery

German Society of Oto-Rhino-Laryngology, Head and Neck Surgery

04.05. - 08.05.2005, Erfurt

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In Vitro Culture of Cells from Oral Mucosa on Foils of Collagen, Poly-L-Lactide (PLLA) and Poly-3-Hydroxy-Butyrate (PHB)

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  • corresponding author Steffen Dommerich - University of Rostock, ENT-Department, Rostock
  • Jürgen Ostwald - University of Rostock, ENT-Department, Rostock
  • Karoline Ziss - University of Rostock, ENT-Department, Rostock
  • Burkhard Kramp - University of Rostock, ENT-Department, Rostock

Deutsche Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie. 76. Jahresversammlung der Deutschen Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie e.V.. Erfurt, 04.-08.05.2005. Düsseldorf, Köln: German Medical Science; 2005. Doc05hno072

The electronic version of this article is the complete one and can be found online at: http://www.egms.de/en/meetings/hno2005/05hno186.shtml

Published: September 22, 2005

© 2005 Dommerich et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.en). You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.

Outline

Text

The replacement of pharyngeal mucosa after surgical resections at different locations in the ENT-field seems to be essential or at least of great benefit for patients.

Cell cultures are initiated mainly by growing out cells from tissue pieces but also by seeding of cells after enzymatic dissociation of mucosa. Cells were cultured with serum-containing and serum-free media on different foils of collagen, Poly-L-Lactid (PLLA) and Poly-hydroxybutyric acid (PHB). Culturing time was on average about 4-5 weeks.

On all tested materials fibroblasts and epithelial cells have grown in principle. PLLA was the material with best properties concerning the aim of this study wheras culturing of cells on PHB only happens after surface modification by aminofunctionalisation with plasma treatment. The cultures were analysed by cell membrane staining and by REM.

In principle the analysed materials are useful for culturing of cells from oral mucosa. PLLA was the material with best properties. However, for obtaining differentiated epithelial cells the culture conditions have to be modified.