Article
Prestimulation of Head and Neck Cancer Cells with Growth Factors effects enhanced Treatment Efficacy
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Published: | September 22, 2005 |
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Background: Treatment of squamous cell carcinomas of the head and neck consists of surgery, radiation and/or chemotherapy. However, despite complete remission of the disease, many patients suffer from recurrences during a follow up period of 2 years.
Methods: Il-6, Serotonin, G-CSF and EGF were used to stimulate G0-phase head and neck cancer cells before treatment with Cisplatin. The proportion of G0-phase cells has been detected by multi color FACS analysis and Ki67 staining.
Results: The combination of highest efficacy to provoke cell cycle reentry has been Serotonin + EGF. This combination has then been used to stimulate cancer cells for chemotherapy with cisplatin. The total amount of G0 phase cells has been significantly reduced after 2-fold Serotonin/EGF coincubation. The prestimulation group showed a significant reduced amount of G0-cells. Furthermore, the total amount of A431, Detroit 562 and UM-SCC 10B cells has been significantly reduced in the prestimulation group compared to the control and cisplatin (without prestimulation) group.
Conclusions: Our investigation demonstrates for the first time that G0 cells of head and neck carcinomas are available for treatment after targeted stimulation with growth factors. This provides a new insight in tumor physiology and should lead to reconsideration of established treatment regimen.