gms | German Medical Science

76th Annual Meeting of the German Society of Oto-Rhino-Laryngology, Head and Neck Surgery

German Society of Oto-Rhino-Laryngology, Head and Neck Surgery

04.05. - 08.05.2005, Erfurt

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Immunohistochemical profiles on a tissue-microarray of 59 malignant parotideal tumors and their impact for tumorigenesis

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  • corresponding author Jürgen Alberty - Klinik und Poliklinik für HNO-Heilkunde, UKM, Münster
  • Heike Tappe - Klinik und Poliklinik für HNO-Heilkunde, UKM, Münster
  • Jens Packeisen - Institute of Pathology, UKM, Münster
  • Horst Bürger - Institute of Pathology, UKM, Münster
  • Wolfgang Stoll - Klinik und Poliklinik für HNO-Heilkunde, UKM, Münster
  • Eberhard Korsching - Institute of Pathology, UKM, Münster
  • Christian August - Institute of Pathology, UKM, Münster

Deutsche Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie. 76. Jahresversammlung der Deutschen Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie e.V.. Erfurt, 04.-08.05.2005. Düsseldorf, Köln: German Medical Science; 2005. Doc05hno666

The electronic version of this article is the complete one and can be found online at: http://www.egms.de/en/meetings/hno2005/05hno145.shtml

Published: September 22, 2005

© 2005 Alberty et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.en). You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.

Outline

Text

Salivary gland tumors are characterized by a wide spectrum of histological and immunohistochemical differentiation. Therefore they are especially suitable to study differences as well as possible common origins in stem cells. Immunohistochemical data of large tumor collective can be further analyzed by means of biomathematical analysis.

Methods: Classification of 59 malignant epithelial parotideal tumors. Immunohistochemical investigation by a tissue microarray-technique; semiquantitative scoring of immunohistochemical findings and correlation by biomathematical cluster analysis.

Results: In relation to tumor entities the immunohistochemical profiles were distributed on four divergent clusters. One cluster was characterized by a strong Cytokeratin 5/6 expression; in this cluster (assumed to represent a more stem-cell associated immunophenotype) only squamous cell carcinomas, mucoepidermoid carcinomas and myoepithelial carcinomas were present. Another cluster was inversely characterized by a strong Cytokeratin 8/18 expression, in this group only salivary duct carcinomas or NOS-adenocarcinomas were present. Two more clusters were characterized by more heterogeneous immunohistochemical profiles. Clusters with Cytokeratin 5/6 (and inversely 8/18) were significantly related to the grade of expression of certain proliferation associated antigens. Such findings may contribute to further classification of salivary gland tumors beyond the borders of classical histological tumor entities.

Conclusions: The results assume – parallel to findings in carcinomas of the female breast - common stem cells to play a role in the tumorigenesis of malignant epithelial tumors of the parotis.