gms | German Medical Science

The ABC Conference: Algae Bioactive Compounds – from research to innovation

The project is funded by Interreg Deutschland-Danmark with means from the European Regional Development Fund.

25. - 26.08.2020, Kiel, Germany (online conference)

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FTIR fingerprinting for fucoidanases' activity assessment

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  • presenting/speaker Vy Ha Nguyen Tran - Department of Bioengineering, Technical University of Denmark, Kongens Lyngby, Denmark; NhaTrang Institute of Technology Research and Application, Vietnam Academy of Science and Technology, Nhatrang, Vietnam
  • Maria Dalgaard Mikkelsen - Department of Bioengineering, Technical University of Denmark, Kongens Lyngby, Denmark
  • Valentina Perna - Department of Bioengineering, Technical University of Denmark, Kongens Lyngby, Denmark
  • Thuan Thi Nguyen - Department of Bioengineering, Technical University of Denmark, Kongens Lyngby, Denmark; NhaTrang Institute of Technology Research and Application, Vietnam Academy of Science and Technology, Nhatrang, Vietnam
  • Vo Thi Dieu Trang - Department of Bioengineering, Technical University of Denmark, Kongens Lyngby, Denmark; NhaTrang Institute of Technology Research and Application, Vietnam Academy of Science and Technology, Nhatrang, Vietnam
  • Andreas Baum - Section for Statistics and Data Analysis, Department of Applied Mathematics and Computer Science, Technical University of Denmark, Kongens Lyngby, Denmark
  • Hang Thi Thuy Cao - NhaTrang Institute of Technology Research and Application, Vietnam Academy of Science and Technology, Nhatrang, Vietnam
  • Tran Thi Thanh Van - NhaTrang Institute of Technology Research and Application, Vietnam Academy of Science and Technology, Nhatrang, Vietnam
  • Anne S. Meyer - Department of Bioengineering, Technical University of Denmark, Kongens Lyngby, Denmark

The FucoSan consortium. The ABC Conference: Algae Bioactive Compounds – from research to innovation. Kiel, 25.-26.08.2020. Düsseldorf: German Medical Science GMS Publishing House; 2020. Doc20fucosan08

doi: 10.3205/20fucosan08, urn:nbn:de:0183-20fucosan087

Published: October 7, 2020

© 2020 Tran et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 License. See license information at http://creativecommons.org/licenses/by/4.0/.

Outline

Text

The cell wall of brown seaweeds contains bioactive compounds such as fucoidans, alginate, polyphenol, peptides, iodine organic products, mannitol, vitamins… Among them, fucoidans have been attractive to study because of their various biological effects in vitro and in vivo such as anticoagulant, antithrombotic, antiviral including anti-HIV, antioxidant [1], and antitumor activity [2]. They essentially contain fucose and sulfate groups with some other groups, such as galactose, xylose, mannose and uronic acids [3], [4]. Fucoidanases with known specificity that catalyzes fucoidan hydrolysis would be a good tool with which to obtain fuco-oligosaccharides without changing the native structure of the fucoidans. Currently, enzymatic action of fucoidanases is measured by state-of-the-art Carbohydrate–Polyacrylamide Gel Electrophoresis (C-PAGE). This method only visualizes substrate alteration or product formation and cannot describe the enzymatic reaction or real time kinetics.

The Fourier Transform Infrared (FTIR) spectroscopy method is a very effective analytical method, with the capability of using infrared spectral signals of substrate consumption and product formation. FTIR is a fast and effective analytical method to evaluate kinetics [5] and quantify activity of different enzymes [6]. In fact, alterations in the molecular structure and interactions can be monitored in the FTIR spectra because the modification in the vibration mode of the molecules involved in the reaction is revealed as spectral changes. The spectral changes induced by the enzymatic reaction can be followed over a period of time. It is the ability to rapidly measure and store an FTIR spectrum, that makes possible, the use of such a methodology in the study of the kinetics of complex reactions [7].

Here we present the spectral evolution of fucoidanase reactions monitored using FTIR. Three different enzymes – FcnAΔ229, FFA2 and Fhf1Δ470 – were evaluated on two different fucoidan substrates from F. evanescens and F. vesiculosus, respectively. The retrieved models, derived from Parallel Factor Analysis (PARAFAC), were used for quantifying the kinetics of the enzyme reaction.

Outline

References

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