gms | German Medical Science

The ABC Conference: Algae Bioactive Compounds – from research to innovation

The project is funded by Interreg Deutschland-Danmark with means from the European Regional Development Fund.

25. - 26.08.2020, Kiel, Germany (online conference)

Functional characterization of an endo-fucoidanase from Formosa haliotis for the degradation of α-(1–3) and α-(1–4) fucoidans

Meeting Abstract

  • presenting/speaker Marlene Vuillemin - Department of Biotechnology and Biomedicine, DTU Bioengineering, Technical University of Denmark, Kongens Lyngby, Denmark
  • Artem S. Silchenko - G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far-Eastern Branch of the Russian Academy of Sciences, Vladivostok, Russia
  • Maxim S. Kokoulin - G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far-Eastern Branch of the Russian Academy of Sciences, Vladivostok, Russia
  • Svetlana P. Ermakova - G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far-Eastern Branch of the Russian Academy of Sciences, Vladivostok, Russia
  • Hang Thi Thuy Cao - Department of Biotechnology and Biomedicine, DTU Bioengineering, Technical University of Denmark, Kongens Lyngby, Denmark; NhaTrang Institute of Technology Research and Application, Vietnam Academy of Science and Technology, Nhatang, Vietnam
  • Trang Vo - Department of Biotechnology and Biomedicine, DTU Bioengineering, Technical University of Denmark, Kongens Lyngby, Denmark; NhaTrang Institute of Technology Research and Application, Vietnam Academy of Science and Technology, Nhatang, Vietnam
  • Jesper Holck - Department of Biotechnology and Biomedicine, DTU Bioengineering, Technical University of Denmark, Kongens Lyngby, Denmark
  • Anne S. Meyer - Department of Biotechnology and Biomedicine, DTU Bioengineering, Technical University of Denmark, Kongens Lyngby, Denmark
  • Maria Dalgaard Mikkelsen - Department of Biotechnology and Biomedicine, DTU Bioengineering, Technical University of Denmark, Kongens Lyngby, Denmark

The FucoSan consortium. The ABC Conference: Algae Bioactive Compounds – from research to innovation. Kiel, 25.-26.08.2020. Düsseldorf: German Medical Science GMS Publishing House; 2020. Doc20fucosan06

doi: 10.3205/20fucosan06, urn:nbn:de:0183-20fucosan063

Published: October 7, 2020

© 2020 Vuillemin et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 License. See license information at http://creativecommons.org/licenses/by/4.0/.


Outline

Text

Fucoidans are complex and heterogeneous sulfated polysaccharides found in the cell wall of brown algae. They present a large variety of structures and have gained an increased interest over the last decades as they can be potentially used for a wide range of applications. Particularly, fucoidan oligosaccharides display an extensive panel of valuable biological activities, such as antioxidant, anticancer, anti-inflammatory, anticoagulant and possess immune-modulatory properties [1].

However, the heterogeneous structure, the viscosity and the large size of the natural fucoidan polysaccharides render them unsuitable for clinical use. Moreover, it is frequently observed that the bioactivity of fucoidans are improved when the molecular weight is lower [2].

Endo-fucoidanases (EC 3.2.1.-) are enzymes that catalyze the hydrolysis of glycosidic bonds between sulfated fucosyl residues in fucoidan polysaccharides [3]. They belong to the CAZy family GH107 of the Glycoside Hydrolases [4]. This quite recent family contains only a very few members up to date: 17 fucoidanases of bacterial origin have been reported in the CAZy database and only six of them have been functionally characterized.

In this study, we aimed to expand the repertoire of known GH107 and identify new tools for the enzymatic synthesis of fucooligosaccharides. We recently identified three genes coding for putative fucoidanases in the genome of the brown-algae-degrading bacterium Formosa haliotis. In this talk, we will present our recent findings on the identification and the characterization of one of the putative fucoidanase named Fhf1. We hope this work will contribute to the better understanding of the structure-function relationships of the GH107 family and will provide new tools for the synthesis of novel active biomolecules.


References

1.
Fitton HJ, Stringer DS, Park AY, Karpiniec SN. Therapies from Fucoidan: New Developments. Mar Drugs. 2019;17(10):571. Published 2019 Oct 9. DOI: 10.3390/md17100571 External link
2.
Song MY, Ku SK, Kim HJ, Han JS. Low molecular weight fucoidan ameliorating the chronic cisplatin-induced delayed gastrointestinal motility in rats. Food Chem Toxicol. 2012;50(12):4468-4478. DOI: 10.1016/j.fct.2012.09.020 External link
3.
Lombard V, Golaconda Ramulu H, Drula E, Coutinho PM, Henrissat B. The carbohydrate-active enzymes database (CAZy) in 2013. Nucleic Acids Res. 2014;42(Database issue):D490-D495. DOI: 10.1093/nar/gkt1178 External link
4.
Colin S, Deniaud E, Jam M, et al. Cloning and biochemical characterization of the fucanase FcnA: definition of a novel glycoside hydrolase family specific for sulfated fucans. Glycobiology. 2006;16(11):1021-1032. DOI: 10.1093/glycob/cwl029 External link