gms | German Medical Science

102. Jahrestagung der DOG

Deutsche Ophthalmologische Gesellschaft e. V.

23. bis 26.09.2004, Berlin

New possibility of amniotic membrane preparation with the water jet

Meeting Abstract

  • corresponding author J.J. Darman - Klinik und Poliklinik für Augenheilkunde der EMA-Universität Greifswald, Greifsald
  • P. Schreier - Klinik und Poliklinik für Augenheilkunde der EMA-Universität Greifswald, Greifsald
  • S. Clemens - Klinik und Poliklinik für Augenheilkunde der EMA-Universität Greifswald, Greifsald
  • F. Tost - Klinik und Poliklinik für Augenheilkunde der EMA-Universität Greifswald, Greifsald

Evidenzbasierte Medizin - Anspruch und Wirklichkeit. 102. Jahrestagung der Deutschen Ophthalmologischen Gesellschaft. Berlin, 23.-26.09.2004. Düsseldorf, Köln: German Medical Science; 2004. Doc04dogFR.19.06

The electronic version of this article is the complete one and can be found online at: http://www.egms.de/en/meetings/dog2004/04dog326.shtml

Published: September 22, 2004

© 2004 Darman et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.en). You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.


Outline

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Objective

Amniotic membrane transplantation is used in the ophthalmology for ocular surface reconstruction. The mechanism of the regeneration of the corneal surface after Amniotic membrane grafting is not well established. This study shows a new technology to remove the epithelial cells from amniotic membrane with the water jet.

Methods

40 Amniotic membrane obtained from caesarean deliveries were prepared under sterile condition in the eye banc of the eye clinic of the Ernst-Moritz-Arndt- university of greifswald. The remove of epithelial cells from the amniotic membrane with the water jet under various pressures, systems, using of aqua dest.. The investigation was performed by light and scanning electron microscopy. One group (10) without polishing and another group (10) after polishing with water jet. We compared the results.

Results

The results show that the epithelial cells of the amniotic membrane were considerably reduced after water jet procedure.

Conclusions

The transplantation of amniotic membrane without epithelial cells appears to be a promising in the surgical treatment for reconstructing the ocular surface.