gms | German Medical Science

27th German Cancer Congress Berlin 2006

German Cancer Society (Frankfurt/M.)

22. - 26.03.2006, Berlin

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Radiation-induced pneumonitis - Identification of high risk groups by analyzing DNA-repair using microgelelectrophoresis (Comet Assay)

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  • corresponding author presenting/speaker Oliver Schneider - Universitätsklinikum Marienhospital, Herne, Deutschland
  • Jürgen Hölzer - Abt. für Hygiene, Umwelt- und Sozialmedizin der Ruhr-Universität Bochum
  • Bindhu Makil - Abt. für Hygiene, Umwelt- und Sozialmedizin der Ruhr-Universität Bochum
  • Marcus Kirnapci - Abt. für Hygiene, Umwelt- und Sozialmedizin der Ruhr-Universität Bochum
  • Michael Wilhelm - Abt. für Hygiene, Umwelt- und Sozialmedizin der Ruhr-Universität Bochum
  • Irenäus A. Adamietz - Universitätsklinikum Marienhospital, Herne

27. Deutscher Krebskongress. Berlin, 22.-26.03.2006. Düsseldorf, Köln: German Medical Science; 2006. DocPO396

The electronic version of this article is the complete one and can be found online at: http://www.egms.de/en/meetings/dkk2006/06dkk506.shtml

Published: March 20, 2006

© 2006 Schneider et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.en). You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.

Outline

Text

Background: The individual risk to develop a radiation-induced pneumonitis can not be exactly predicted. Co-factors are known, however predictive tests have no clinical importance. The following study analyses, if the individual radiation sensitivity can be better characterized by DNA-repair in peripheral lymphocytes (PL) using microgelectrophoresis (MGE, Comet Assay).

Patients and Methods: PL were isolated from venous blood samples of patients undergoing radiotherapy because of lung cancer (n=37) und of healthy subjects. PL were radiated in vitro (10 MeV; 5 Gy). Aliquots of radiated and non-radiated PL were analysed measuring DNA-strand breaks and alkali-labile areas using MGE (0-120 min. and 24 hrs. after radiation). All patients who survived more than 3 months were evaluated. To quantificate DNA-repair, quotients of median of the Tail Extent Moments (Komet® 4, Kinetic Imaging) were built.

Results: Significant interindividual differences in the frequency of DNA-strand-breaks and DNA-repair were detected. 17 patients demonstrated a RP in the CT. RP was analysed in 100 % of the patients in the group with high radiation sensitivity (>75-percentile), in 82% with medium radiation sensitivity (25-75-perzentile) and in 50 % in the group of low radiation sensitivity.

Conclusion: The Comet-Assay is able to detect interindividual differences of reparation capacity after irradiation und might be a help to assess the individual risk to develop a radiation-induced pneumonitis.