gms | German Medical Science

35. Kongress der Deutschen Kontinenz Gesellschaft

Deutsche Kontinenz Gesellschaft e. V.

22.11. - 23.11.2024, Essen

Highly Concentrated β-Sitosterol Inhibits Human Prostate Smooth Muscle Contraction: The Rise of Phytotherapy in LUTS/BPH

Meeting Abstract

Deutsche Kontinenz Gesellschaft e.V.. 35. Kongress der Deutschen Kontinenz Gesellschaft. Essen, 22.-23.11.2024. Düsseldorf: German Medical Science GMS Publishing House; 2024. Doc43

doi: 10.3205/24dkg43, urn:nbn:de:0183-24dkg430

Published: November 20, 2024

© 2024 Tamalunas et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 License. See license information at http://creativecommons.org/licenses/by/4.0/.


Outline

Text

Introduction: Recently, DGU and EAU recommended phytotherapeutics in their guidelines on management of non-neurogenic male LUTS [1]. While production techniques and plant extracts differ between manufacturers and their countries of origin, phytosterols seem to be the smallest common denominator of phytotherapeutics available for LUTS/BPH [1], [2]. We therefore investigated the effects of highly-concentrated phytosterol (β-Sitosterol) on human prostate smooth muscle contraction.

Methods: Apoprostat® forte capsules (>80% β-Sitosterol) were dissolved in ethanol (10ml ethanol/capsule). Prostate tissues were obtained from patients undergoing radical prostatectomy for prostate cancer (n=100 patients). Contractility of tissue strips was assessed in an organ bath and normalized to KCl [1], [2], [3]. Percentages of inhibition reflect area under the curve (AUC) for treatment vs. solvent.

Results: Apoprostat® forte (30, 10, and 3µg/ml) concentration-dependently inhibited α1-adrenergic contractions up to 57%, 71%, and 69% for noradrenaline (A-C), phenylephrine (D-F), and methoxamine (G-I), and non-adrenergic contractions up to 69% and 46% for thromboxane A2-analogue U46619 (J-L) and endothelin-1 (M-O) vs. control (ethanol), respectively. Electric field stimulation-induced neurogenic contractions were inhibited up to 63% (P-R).

Figure 1 [Fig. 1]

Conclusion: Phytoestrogen β-Sitosterol inhibits prostatic contraction, which may contribute to low prevalence of LUTS/BPH in Asia [2]. Using the highest concentrate of β-Sitosterol available on the German market, our results provide a powerful basis explaining the in-vivo-efficacy of phytotherapeutics for LUTS/BPH [1].

Conflict of interest: As part of study participation, paid lectures and advisory board activities, the authors have contacts with the following companies:

  • Merck
  • Trovagene
  • MSD
  • Pfizer
  • Apogepha
  • Cardiff Oncology

References

1.
Tamalunas A, Wendt A, Springer F, Vigodski V, Ciotkowska A, Rutz B, Wang R, Huang R, Liu Y, Schulz H, Ledderose S, Kolben T, Magistro G, Stief CG, Hennenberg M. Permixon®, hexane-extracted Serenoa repens, inhibits human prostate and bladder smooth muscle contraction and exerts growth-related functions in human prostate stromal cells. Life Sci. 2022 Nov 1;308:120931. DOI: 10.1016/j.lfs.2022.120931 External link
2.
Huang R, Liu Y, Hu S, Tamalunas A, Waidelich R, Strittmatter F, Stief CG, Hennenberg M. Inhibition of alpha1-Adrenergic, Non-Adrenergic and Neurogenic Human Prostate Smooth Muscle Contraction and of Stromal Cell Growth by the Isoflavones Genistein and Daidzein. Nutrients. 2022 Nov 22;14(23):4943. DOI: 10.3390/nu14234943 External link
3.
Tamalunas A, Wendt A, Springer F, Ciotkowska A, Rutz B, Wang R, Huang R, Liu Y, Schulz H, Ledderose S, Magistro G, Stief CG, Hennenberg M. Inhibition of Human Prostate and Bladder Smooth Muscle Contraction, Vasoconstriction of Porcine Renal and Coronary Arteries, and Growth-Related Functions of Prostate Stromal Cells by Presumed Small Molecule Gaq/11 Inhibitor, YM-254890. Front Physiol. 2022 May 23;13:884057. DOI: 10.3389/fphys.2022.884057 External link