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Deutscher Rheumatologiekongress 2023

51. Kongress der Deutschen Gesellschaft für Rheumatologie (DGRh)
37. Jahrestagung der Deutschen Gesellschaft für Orthopädische Rheumatologie (DGORh)
33. Jahrestagung der Gesellschaft für Kinder- und Jugendrheumatologie (GKJR)

30.08. - 02.09.2023, Leipzig

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Pathophysiological relevance of B Cell Activating Factor (BAFF) and BAFF Receptor (BAFF-R) for renal ischemia/reperfusion (I/R) injury

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  • Tamara Möckel - University Medical Center of the Johannes Gutenberg University Mainz, Department of Internal Medicine I, Division of Rheumatology and Clinical Immunology, Mainz
  • Sebastian Boegel - University Medical Center of the Johannes Gutenberg University Mainz, Department of Internal Medicine I, Division of Rheumatology and Clinical Immunology, Mainz
  • Andreas Schwarting - University Medical Center of the Johannes Gutenberg University Mainz, Department of Internal Medicine I, Division of Rheumatology and Clinical Immunology, Mainz; Rheumatology Center Rhineland Palatinate, Bad Kreuznach

Deutsche Gesellschaft für Rheumatologie. Deutsche Gesellschaft für Orthopädische Rheumatologie. Gesellschaft für Kinder- und Jugendrheumatologie. Deutscher Rheumatologiekongress 2023, 51. Kongress der Deutschen Gesellschaft für Rheumatologie (DGRh), 37. Jahrestagung der Deutschen Gesellschaft für Orthopädische Rheumatologie (DGORh), 33. Jahrestagung der Gesellschaft für Kinder- und Jugendrheumatologie (GKJR). Leipzig, 30.08.-02.09.2023. Düsseldorf: German Medical Science GMS Publishing House; 2023. DocET.14

doi: 10.3205/23dgrh034, urn:nbn:de:0183-23dgrh0343

Published: August 30, 2023

© 2023 Möckel et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 License. See license information at http://creativecommons.org/licenses/by/4.0/.

Outline

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Introduction: Acute kidney injury (AKI) accompanies with high morbidity and mortality. Incomplete renal recovery can lead to chronic and finally end-stage kidney disease, which results in the requirement of lifelong dialysis or kidney transplantation. The B cell activating factor (BAFF), a cytokine of the tumor necrosis factor (TNF) family, plays an important role for activation, differentiation and survival of B cells. By supporting B cells, which in turn play an important role in inflammatory processes and the production of antibodies, BAFF is related to AKI, transplantation and graft rejection.

Methods: In order to investigate the impact of BAFF and its receptor BAFF-R on the development of AKI, we performed the well-established ischemia/reperfusion (I/R) model in BAFF (B6.129S2-Tnfsf13btm1Msc/J) and BAFF-R (B6(Cg)-Tnfrsf13ctm1Mass/J) deficient mice. To receive a first impression of the effect of BAFF and BAFF-R knockout, the transcriptome of the ischemic and contralateral control kidneys was analyzed and compared to the wildtype littermates.

Results: In ischemic kidneys, we detected the upregulation of the genes Lcn2, Lyz2, Cd44, Fn1 and Il1rn as well as the downregulation of Kl with different expression patterns in BAFF and BAFF-R knockout mice. Up- and downregulation of each investigated gene was higher in BAFF-R knockout and lower in BAFF knockout compared to wildtype littermates. Furthermore, Lysozyme 2 (Lyz2) expression was remarkable upregulated in ischemic kidneys in both strains.

Conclusion: Findings of this study lead to the assumption that BAFF knockout seems to have a positive effect, while BAFF-R knockout worsened I/R injury. In addition, our study shows for the first time a remarkable renal upregulation of Lyz2 in a murine I/R model. Therefore, Lyz2 can be a conceivable predictive or early biomarker in case of I/R and AKI.