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43. Kongress der Deutschen Gesellschaft für Rheumatologie, 29. Jahrestagung der Deutschen Gesellschaft für Orthopädische Rheumatologie, 25. Wissenschaftliche Jahrestagung der Gesellschaft für Kinder- und Jugendrheumatologie

02.-05. September 2015, Bremen

Elevated immunosenescence parameters of CD8+ T cells in CMV-seropositive

Meeting Abstract

  • Giovanni Almanzar - Universitätsklinikum Würzburg, Kinderklinik, Würzburg
  • Marc Schmalzing - Universitätsklinikum Würzburg, Medizinische Klinik und Poliklinik II, Schwerpunkt Rheumatologie und klinische Immunologie, Würzburg
  • Raimund Trippen - Laboratory of Pediatric Rheumatology, Special Immunology and Osteology, Department of Pediatrics, University of Würzburg, Würzburg
  • Kerstin Höfner - Universitätsklinikum Würzburg, Kinderklinik, Würzburg
  • Benedikt Weißbrich - Institut für Virologie und Immunbiologie, Würzburg
  • Eva Geissinger - Institute of Pathology, University of Wuerzburg, Wuerzburg, Germany, Würzbrug
  • Thomas Meyer - Department of Surgery, University of Würzburg, Würzburg, Germany, Würzburg
  • Johannes Liese - Universitäts-Kinderklinik, Würzburg
  • Hans-Peter Tony - Universitätsklinikum Würzburg, Medizinische Klinik und Poliklinik II, Schwerpunkt Rheumatologie und klinische Immunologie, Würzburg
  • Martina Prelog - Universitätsklinikum Würzburg, Kinderklinik, Würzburg

Deutsche Gesellschaft für Rheumatologie. Deutsche Gesellschaft für Orthopädische Rheumatologie. Gesellschaft für Kinder- und Jugendrheumatologie. 43. Kongress der Deutschen Gesellschaft für Rheumatologie (DGRh); 29. Jahrestagung der Deutschen Gesellschaft für Orthopädische Rheumatologie (DGORh); 25. wissenschaftliche Jahrestagung der Gesellschaft für Kinder- und Jugendrheumatologie (GKJR). Bremen, 02.-05.09.2015. Düsseldorf: German Medical Science GMS Publishing House; 2015. DocRA.39

doi: 10.3205/15dgrh205, urn:nbn:de:0183-15dgrh2055

Published: September 1, 2015

© 2015 Almanzar et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 License. See license information at



Introduction: Latent Cytomegalovirus (CMV) infection drives immunosenescence in elderly showing increased CD28- T cells, enhanced peripheral T cell proliferation, TCR repertoire restriction and aberrant IFNγ responses. Reactivations of CMV and abnormal responses towards CMV have been reported in rheumatoid arthritis (RA) in juvenile idiopathic arthritis (JIA).

Methods: To evaluate the associations between latent CMV infection and immunosenescence parameters in autoimmune arthritis, unspecific and CMVpp65-specific IFNgamma responses were analyzed in peripheral CD8+ T cells in 57 RA or 60 JIA patients compared to healthy, age-matched controls by ELISPOT and flow cytometry.

Results: Higher prevalence and concentrations of IgG-anti-CMV were found in RA. RA and JIA patients showed lower unspecific IFNgamma production, lower CD69-mediated activation and lower Ki67+ CD8+ T cell proliferation, but higher intracellular IFNgamma production and increased proportions of CD28-negative T cells after specific CMVpp65 stimulation. CMVpp65-specific cellular reactivity was not altered by blockade of IL-6 or TNFalpha in vitro. JIA patients showed a skewed TCR repertoire towards oligoclonality and less polyclonality.

Conclusion: Our results suggest an efficient control of latent CMV infection by CMVpp65-specific IFNgamma production with expansion of CD28- CD8+ T cells, although lower unspecific intracellular IFNgamma production and peripheral CD8+ T cell activation, as well as proliferation and TCR diversity restriction were found in CMV-seropositive patients with autoimmune arthritis. In vitro blockade of TNFalpha or IL-6 seems not to influence CMVpp65-specific cellular reactivity, but may be studied in the long-term with translation to therapeutic effects of biologics on the need for life-long immunological control of CMV and inflammation.