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43. Kongress der Deutschen Gesellschaft für Rheumatologie, 29. Jahrestagung der Deutschen Gesellschaft für Orthopädische Rheumatologie, 25. Wissenschaftliche Jahrestagung der Gesellschaft für Kinder- und Jugendrheumatologie

02.-05. September 2015, Bremen

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The differential impact of TH+ neuronal cell therapy in models of experimental arthritis

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  • Matthias Ebbinghaus - Universitätsklinikum Jena, Institut für Physiologie I, Jena
  • Zsuzsa Jenei-Lanzl - Universitätsklinikum Regensburg, Klinik und Poliklinik für Innere Medizin I, Rheumatologie und Klinische Immunologie, Regensburg
  • Rainer H. Straub - Innere Med I, Universitätsklinikum Regensburg, Regensburg
  • Hans-Georg Schaible - Universitätsklinikum Jena, Institut für Physiologie I, Jena

Deutsche Gesellschaft für Rheumatologie. Deutsche Gesellschaft für Orthopädische Rheumatologie. Gesellschaft für Kinder- und Jugendrheumatologie. 43. Kongress der Deutschen Gesellschaft für Rheumatologie (DGRh); 29. Jahrestagung der Deutschen Gesellschaft für Orthopädische Rheumatologie (DGORh); 25. wissenschaftliche Jahrestagung der Gesellschaft für Kinder- und Jugendrheumatologie (GKJR). Bremen, 02.-05.09.2015. Düsseldorf: German Medical Science GMS Publishing House; 2015. DocER.24

doi: 10.3205/15dgrh078, urn:nbn:de:0183-15dgrh0786

Published: September 1, 2015

© 2015 Ebbinghaus et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 License. See license information at http://creativecommons.org/licenses/by/4.0/.

Outline

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Introduction: A long-lasting anti-inflammatory effect of generated catecholamine-producing tyrosine hydroxylase-positive (TH+) cells was shown in collagen type II-induced arthritis (CIA). Here, we investigated the importance of TH+ cells in another model of rheumatoid arthritis.

Methods: TH+ cells were generated from murine bone -derived mesenchymal stem cells by specific catecholaminergic factors. Antigen-induced arthritis (AIA) was induced in C57BL/6J mice and one group was treated by TH+ cell-transfer. The appearance of natural TH+ cells, clinical signs of arthritis, pain and immune parameters were observed during AIA.

Results: In contrast to CIA, here, only a small number of natural occurring TH+ cells were detectable in spleen in the acute phase of arthritis. After TH+ cell transfer, joint swelling was only moderately reduced in acute AIA, but mice showed significantly less guarding and less reduction of withdrawal threshold for mechanical stimulation on the inflamed hind limb. These effects were attenuated towards later phases of the disease. Furthermore both, in TH+ treated mice cytokines in knee joint extracts and immunoglobulins and cytokines in sera were not reduced in acute AIA. Moreover, significantly enhanced values of IL-17, IgG1 and IgG3 were detected.

Conclusion: In acute AIA, TH+ cells have rather an anti-nociceptive than an anti-inflammatory effect. The increase of inflammatory parameters corresponds to the effect of the sympathetic system on the acute phase of the AIA.