gms | German Medical Science

50. Jahrestagung der Deutschen Gesellschaft der Plastischen, Rekonstruktiven und Ästhetischen Chirurgen (DGPRÄC), 24. Jahrestagung der Vereinigung der Deutschen Ästhetisch-Plastischen Chirurgen (VDÄPC)

26.09. - 28.09.2019, Hamburg

A novel 3D cell culture model of tumor angiogenesis to study soft tissue sarcomas (STS)

Meeting Abstract

  • presenting/speaker Xeni Delvinioti - Otto-von-Guericke Universität, Magdeburg
  • Maximilian Kückelhaus - Uniklinikum Magdeburg, Plastische, Ästhetische und Handchirurgie, Magdeburg; Universität, Plastische Chirurgie, Münster; Fachklinik Hornheide, Plastische Chirurgie, Münster
  • Marcus Lehnhardt - Uniklinikum Magdeburg, Plastische, Ästhetische und Handchirurgie, Magdeburg; Universität, Plastische Chirurgie, Münster; Fachklinik Hornheide, Plastische Chirurgie, Münster; Bergmannsheil, Plastische Chirurgie, Bochum
  • Frank Jacobsen - Uniklinikum Magdeburg, Plastische, Ästhetische und Handchirurgie, Magdeburg; Universität, Plastische Chirurgie, Münster; Fachklinik Hornheide, Plastische Chirurgie, Münster; Bergmannsheil, Plastische Chirurgie, Bochum
  • Manfred Infanger - Uniklinikum Magdeburg, Plastische, Ästhetische und Handchirurgie, Magdeburg; Universität, Plastische Chirurgie, Münster; Fachklinik Hornheide, Plastische Chirurgie, Münster; Bergmannsheil, Plastische Chirurgie, Bochum
  • Tobias Hirsch - Uniklinikum Münster, Plastische Chirurgie, Münster

Deutsche Gesellschaft der Plastischen, Rekonstruktiven und Ästhetischen Chirurgen. Vereinigung der Deutschen Ästhetisch-Plastischen Chirurgen. 50. Jahrestagung der Deutschen Gesellschaft der Plastischen, Rekonstruktiven und Ästhetischen Chirurgen (DGPRÄC), 24. Jahrestagung der Vereinigung der Deutschen Ästhetisch-Plastischen Chirurgen (VDÄPC). Hamburg, 26.-28.09.2019. Düsseldorf: German Medical Science GMS Publishing House; 2019. Doc176

doi: 10.3205/19dgpraec176, urn:nbn:de:0183-19dgpraec1765

Published: September 24, 2019

© 2019 Delvinioti et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 License. See license information at http://creativecommons.org/licenses/by/4.0/.


Outline

Text

Background: Potential personalized therapeutic strategies introducing novel diagnostic methods are crucial in order to identify the aggressiveness of STS. It is well known that tumor angiogenesis is essential for tumor growth and metastasis. In the current study we established a novel 3D cell culture model in order to observe the tumor progression and the angiogenic potential of these malignant neoplasms in vitro.

Methods: We used three different human STS cell lines, the EGFP stably-expressing HT1080 cells (fibrosarcoma), the SW872 (liposarcoma) cells as well the Syo1 (synovial sarcoma) cells in co-culture with normal dermal fibroblasts or tumor associated fibroblasts and the co-spheroids, embedded in a fibrin matrix containing endothelial cells. We compared the effects of dermal fibroblasts and tumor associated fibroblasts on the development of STS. We used an Alamar Blue Assay, a Scratch Test and a Matrigel Assay to detect how the fibroblasts affect the proliferation rate, the migration and invasion ability of cancer cells, respectively. Additionally, to investigate the angiogenic potential, we analyzed via immunohistochemistry the protein expression of established markers such as CD31, a-SMA, VEGF and PDGF. Furthermore, using quantitative PCR, we analyzed the relative expression of genes related to mesenchymal features, angiogenic and metastatic potential and stemness.

Results: Our 3D cell culture model was functional and efficient for all three tested cell lines. Specifically, we observed growth of vessel-like sprouting within 1–3 days. The dermal fibroblasts appear to have a myofibroblastic phenotype as well as stemness. The dermal fibroblasts reduce the proliferation rate of cancer cells but they seem to positively influence their invasion ability.

Conclusion: We have established a novel 3D cell culture model that will broaden our ability to study soft tissue sarcomas and will enable the development of efficient strategies for personalized therapy.