gms | German Medical Science

50. Jahrestagung der Deutschen Gesellschaft der Plastischen, Rekonstruktiven und Ästhetischen Chirurgen (DGPRÄC), 24. Jahrestagung der Vereinigung der Deutschen Ästhetisch-Plastischen Chirurgen (VDÄPC)

26.09. - 28.09.2019, Hamburg

Multiple symmetric lipomatosis: First histological and immunohistochemical characterization of adipocytes from affected and unaffected tissue of the same patient

Multiple Symmetrische Lipomatose: Erste histologische und immunhistochemische Charakterisierung von Adipozyten aus betroffenem und unbetroffenem Gewebeder gleichen Patienten

Meeting Abstract

  • presenting/speaker Daniel Schiltz - Universitätsklinikum Regensburg, Regensburg
  • Sebastian Tschernitz - Universitätsklinikum Regensburg, Dermatologie, Regensburg
  • Christine Ortner - Universitätsklinikum Regensburg, Dermatologie, Regensburg
  • Alexandra Anker - Universitätsklinikum Regensburg, Plastische Chirurgie, Regensburg
  • Silvan Klein - Universitätsklinikum Regensburg, Plastische Chirurgie, Regensburg
  • Oliver Felthaus - Universitätsklinikum Regensburg, Plastische Chirurgie, Regensburg
  • Lukas Prantl - Universitätsklinikum Regensburg, Plastische Chirurgie, Regensburg
  • Stephan Schreml - Universitätsklinikum Regensburg, Dermatologie, Regensburg

Deutsche Gesellschaft der Plastischen, Rekonstruktiven und Ästhetischen Chirurgen. Vereinigung der Deutschen Ästhetisch-Plastischen Chirurgen. 50. Jahrestagung der Deutschen Gesellschaft der Plastischen, Rekonstruktiven und Ästhetischen Chirurgen (DGPRÄC), 24. Jahrestagung der Vereinigung der Deutschen Ästhetisch-Plastischen Chirurgen (VDÄPC). Hamburg, 26.-28.09.2019. Düsseldorf: German Medical Science GMS Publishing House; 2019. Doc132

doi: 10.3205/19dgpraec132, urn:nbn:de:0183-19dgpraec1320

Published: September 24, 2019

© 2019 Schiltz et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 License. See license information at http://creativecommons.org/licenses/by/4.0/.


Outline

Text

Multiple symmetric lipomatosis (MSL) (syn.: Launois Bençaude Syndrome, Morbus Madelung, benign symmetric lipomatosis) is a rare disease of fat tissue. The pathophysiology of MSL still remains unclear, although several approaches have been described. Beside morphologic characteristics and some molecular cell biological approaches, there is less known about the histological characterization of adipocytes from MSL-affected patients. Especially a systematic histological and immunohistological workup and comparison of affected and unaffected adipose tissue in patients with MSL has never been done before.

Between 2011 and 2017 we found 45 patients with diagnosed MSL in the database of the University Hospital Regensburg. From 10 patients, fat tissue samples were collected in local anaesthesia from affected and unaffected areas (with patient approval). As unaffected area, the forearm was chosen, as this area is never affected from adipose tissue hyperplasia in MSL patients. After fixation in 4% formaldehyde solution, the sections were analysed by electronic microscope and the following stainings: Hämatoxylin-Eosin, Elastik van Geison, Ladewig, CD 200, CIDEA, myf5, p107, Prdm16, Sca-1, Syndecan, UCP1, MAC387, Glut4.

In patients suffering from MSL, no macroscopicle and microscopicle difference could be found between affected and unaffected adipose tissue. In the affected adipose tissue, 9 patients were positivity for UCP1. In 4 assays a partially very weak positivity for myf5 was found in the affected adipose tissue. The other stainings showed no difference between affected and unaffected adipocytes.

We consider the proliferation of adipose tissue as a tissue hyperplasia rather than a tissue hypertrophy. The expression of myf5 in the examined samples supports the hypothesis that affected adipose tissue derives from white or beige adipose tissue rather than from brown fat.