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50. Jahrestagung der Deutschen Gesellschaft der Plastischen, Rekonstruktiven und Ästhetischen Chirurgen (DGPRÄC), 24. Jahrestagung der Vereinigung der Deutschen Ästhetisch-Plastischen Chirurgen (VDÄPC)

26.09. - 28.09.2019, Hamburg

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Leptin promotes the survival of adipocytes after non-vascularized fat grafting

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  • presenting/speaker Jeannine Schreiter - Universitätsklinikum Leipzig, Leipzig
  • Olga Kurow - Universitätsklinikum Leipzig, Orthopädie, Unfallchirurgie und Plastische Chirurgie, Leipzig
  • Nora Klöting - Universitätsklinikum Leipzig, IFB Adipositas-Erkrankungen, Leipzig
  • Christian Beescho - Universitätsklinikum Leipzig, Orthopädie, Unfallchirurgie und Plastische Chirurgie, Leipzig
  • Stefan Langer - Universitätsklinikum Leipzig, Orthopädie, Unfallchirurgie und Plastische Chirurgie, Leipzig

Deutsche Gesellschaft der Plastischen, Rekonstruktiven und Ästhetischen Chirurgen. Vereinigung der Deutschen Ästhetisch-Plastischen Chirurgen. 50. Jahrestagung der Deutschen Gesellschaft der Plastischen, Rekonstruktiven und Ästhetischen Chirurgen (DGPRÄC), 24. Jahrestagung der Vereinigung der Deutschen Ästhetisch-Plastischen Chirurgen (VDÄPC). Hamburg, 26.-28.09.2019. Düsseldorf: German Medical Science GMS Publishing House; 2019. Doc123

doi: 10.3205/19dgpraec123, urn:nbn:de:0183-19dgpraec1235

Published: September 24, 2019

© 2019 Schreiter et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 License. See license information at http://creativecommons.org/licenses/by/4.0/.

Outline

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Background/Purpose: Autologous fat transplantation is frequently performed in plastic surgery for soft tissue augmentation. The survival of the fat graft after implantation is the primordial goal and the vascularization of the graft is essential for this. Nevertheless, little is known of the adipose tissue graft vascularization and development of adipocytes immediately after transplantation. The aim of this study was to assess the effect of the angiogenesis-promoting hormone leptin on the angiogenesis of the graft, the survival, size and morphology of the adipocytes.

Methods: In a murine model, inguinal fat was autologously transplanted into a dorsal skinfold chamber. After implantation into the dorsal skinfold chamber transplants were treated with leptin or saline. At four different postoperative days (day 3, 7, 10, 15) the vascularization of the graft, the course of angiogenesis and adipocytes’ morphology was assessed. Post-mortem analyses including immunohistochemistry with anti-Caspase-3 and -Perilipin antibody were performed.

Results: The vascularization of the adipose tissue implant started from the transplants’ border zone in both groups. The perfused fat graft area was significantly higher in the leptin group. Morphology of adipocytes changed from polygonal to round cells in all groups starting from day 7. Histologic examination revealed reduced number of caspase-3 positive cells in the leptin group compared to the control. The percentage of the perilipin positive area of the fat graft was significantly higher in the leptin group.

Conclusions: Our findings may suggest that Leptin can effectively promote the survival, neovascularization and attenuated apoptosis in the fat grafts after nonvascularized fat graft implantation.