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45. Jahrestagung der Deutschen Gesellschaft der Plastischen, Rekonstruktiven und Ästhetischen Chirurgen (DGPRÄC), 19. Jahrestagung der Vereinigung der Deutschen Ästhetisch-Plastischen Chirurgen (VDÄPC), 52. Jahrestagung der Österreichischen Gesellschaft für Plastische, Ästhetische und Rekonstruktive Chirurgie (ÖGPÄRC)

11.09. - 13.09.2014, München

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Inactivation of Acetinobacter baumanii growth in a third-degree burn mouse model using Irreversible Electroporation (IRE)

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  • presenting/speaker G. Felix Brölsch - Med. Hochschule Hannover, Plastische, Hand- und Wiederherstellungschirurgie, Hannover, Deutschland; Massachusetts General Hospital, Department of Surgery, Boston, Vereinigte Staaten Von Amerika
  • Alex Golberg - Massachusetts General Hospital, Department of Surgery, Boston, Vereinigte Staaten von Amerika
  • Daniela Vecchio - Massachusetts General Hospital, Wellman Center for Photomedicine, Boston, Vereinigte Staaten von Amerika
  • Saiqa Khan - Massachusetts General Hospital, Division of Plastic and Reconstructive Surgery, Boston, Vereinigte Staaten von Amerika
  • William G. Austen - Massachusetts General Hospital, Division of Plastic and Reconstructive Surgery, Boston, Vereinigte Staaten von Amerika
  • Robert L. Sheridan - Massachusetts General Hospital, Department of Surgery, Boston, Vereinigte Staaten von Amerika
  • Martin L. Yarmush - Massachusetts General Hospital, Department of Surgery, Boston, Vereinigte Staaten von Amerika
  • Michael R. Hamblin - Massachusetts General Hospital, Wellman Center for Photomedicine, Boston, Vereinigte Staaten von Amerika

Deutsche Gesellschaft der Plastischen, Rekonstruktiven und Ästhetischen Chirurgen. Vereinigung der Deutschen Ästhetisch-Plastischen Chirurgen. Österreichische Gesellschaft für Plastische, Ästhetische und Rekonstruktive Chirurgie. 45. Jahrestagung der Deutschen Gesellschaft der Plastischen, Rekonstruktiven und Ästhetischen Chirurgen (DGPRÄC), 19. Jahrestagung der Vereinigung der Deutschen Ästhetisch-Plastischen Chirurgen (VDÄPC), 52. Jahrestagung der Österreichischen Gesellschaft für Plastische, Ästhetische und Rekonstruktive Chirurgie (ÖGPRÄC). München, 11.-13.09.2014. Düsseldorf: German Medical Science GMS Publishing House; 2014. Doc282

doi: 10.3205/14dgpraec087, urn:nbn:de:0183-14dgpraec0872

Published: September 3, 2014

© 2014 Brölsch et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.en). You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.

Outline

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Objective: Emerging bacterial resistance renders many antibiotics ineffective, making alternative strategies of wound disinfection important. Here we report on a new, physical burn wound disinfection method: irreversible electroporation (IRE). High voltage, short-pulsed electric fields (PEF) create non-thermal, permanent damage to cell membranes. In medicine, IRE has recently been used for non-thermal ablation of solid tumors. We have expanded the spectrum of IRE applications in medicine to the treatment of burn wounds.

Methods: A third-degree burn was induced on the dorsal skin of C57BL/6 mice. Immediately after the injury, the burn wound was infected with Acinetobacter baumannii expressing luxCDABE operon. Thirty minutes after infection, the infected areas were treated with 80 pulses delivered at 500 Vmm-1, 70µs, 1Hz. We used bioluminescence to quantify bacteria on skin. Three animals were used per experimental condition.

Results: IRE was effective in the disinfection of infected burn murine skin. The bacteria load reduction was correlated with the number of delivered pulses. Forty pulses of Vmm-1 led to 2.04 ± 0.29 Log10 reduction in bacteria load; 80 pulses led to the immediate 5.53 ± 0.30 Log10 reduction. Three hours after PEF, the bacteria reduction on the skin treated by 500 Vmm-1, 80 pulses was 4.91 ± 0.71 Log10.

Conclusions: We introduce a new method of wound disinfection using high voltage, short-PEF. We believe that IRE may represent an important alternative to antibiotics in addressing bacterial contamination of wounds, particularly those contaminated with multi-drug resistant bacteria.

Figure 1 [Fig. 1], Figure 2 [Fig. 2]