Article
Evaluation of morphology and behaviour of microglia obtained from human glioma
Evaluation der Morphologie und des Verhaltens der Mikrogliazellen aus humanen Gliomen
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Published: | June 4, 2021 |
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Objective: Microglia, mono-nuclear cells distributed throughout the CNS, are key immune effector cells of the brain. The complex interactions between microglia and the other cell types in glioma play a crucial role in the development of novel treatments, presumably via the regulation of tumor progression and anti-tumor immune responses. This study was aimed to evaluate the morphology and behavior of microglia isolated from human glioma and compare these characteristics with microglia obtained from epileptic brain tissues.
Methods: The morphology, expression of the microglia-specific binding protein, migration ability, phagocytosis, apoptosis, and the expression of excitatory as well as inhibitory receptors were assessed.
Results: Our preliminary findings have shown a wide range of various morphologies of both activated- and non-activated microglia associated with strong spatiotemporal alterations in morphology over 4 weeks of cell culture. Furthermore, different forms of microglia exhibited different patterns of migration ability. Various patterns of the microglia-specific binding protein, as well as glutamate and GABA receptor expression, were identified in microglia with different morphologies. Microglia with different morphologies exerted different phagocytic properties. The behavior and morphology of microglia obtained from glioblastoma tissues were markedly different from those from epileptic brain tissues.
Conclusion: Our findings provide comprehensive information on the behavior of microglia in glioma, setting the basis for a more detailed classification and new insights for the designation of novel glioma treatment via targeting microglia.