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72. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie (DGNC)
Joint Meeting mit der Polnischen Gesellschaft für Neurochirurgie

Deutsche Gesellschaft für Neurochirurgie (DGNC) e. V.

06.06. - 09.06.2021

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Variants of oxidised regenerated cellulose cause different cellular damage on organotypic hippocampal slice cultures

Verschiedene Varianten oxidierter regenerierter Zellulose verursachen ein unterschiedliches Ausmaß an Zellschäden bei organotypischen Hippocampus-Schnittkulturen

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  • presenting/speaker Joshua Kleine - Martin-Luther-Universität Halle-Wittenberg, Institut für Anatomie und Zellbiologie, Medizinische Fakultät, Halle/Saale, Deutschland
  • presenting/speaker Sandra Leisz - Martin-Luther-Universität Halle-Wittenberg, Klinik für Neurochirurgie, Medizinische Fakultät, Halle/Saale, Deutschland
  • Chalid Ghadban - Martin-Luther-Universität Halle-Wittenberg, Institut für Anatomie und Zellbiologie, Medizinische Fakultät, Halle/Saale, Deutschland
  • Tim Hohmann - Martin-Luther-Universität Halle-Wittenberg, Institut für Anatomie und Zellbiologie, Medizinische Fakultät, Halle/Saale, Deutschland
  • Christian Scheller - Martin-Luther-Universität Halle-Wittenberg, Klinik für Neurochirurgie, Medizinische Fakultät, Halle/Saale, Deutschland
  • Julian Prell - Martin-Luther-Universität Halle-Wittenberg, Klinik für Neurochirurgie, Medizinische Fakultät, Halle/Saale, Deutschland
  • Christian Strauss - Martin-Luther-Universität Halle-Wittenberg, Klinik für Neurochirurgie, Medizinische Fakultät, Halle/Saale, Deutschland
  • Faramarz Dehghani - Martin-Luther-Universität Halle-Wittenberg, Institut für Anatomie und Zellbiologie, Medizinische Fakultät, Halle/Saale, Deutschland
  • Sebastian Simmermacher - Martin-Luther-Universität Halle-Wittenberg, Klinik für Neurochirurgie, Medizinische Fakultät, Halle/Saale, Deutschland

Deutsche Gesellschaft für Neurochirurgie. 72. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie (DGNC), Joint Meeting mit der Polnischen Gesellschaft für Neurochirurgie. sine loco [digital], 06.-09.06.2021. Düsseldorf: German Medical Science GMS Publishing House; 2021. DocV207

doi: 10.3205/21dgnc202, urn:nbn:de:0183-21dgnc2026

Published: June 4, 2021

© 2021 Kleine et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 License. See license information at http://creativecommons.org/licenses/by/4.0/.

Outline

Text

Objective: Hemostasis is one of the challenging problems in neurosurgery. Based on oxidized regenerated cellulose (ORC) several hemostyptic materials like Tabotamp®, Equicel® and Equitamp® have been developed. The present study compared ORC that differ in their compositions and properties. In organotypic hippocampal slice cultures (OHSC) the cytotoxicity and damage on neuronal tissue was evaluated.

Methods: Properties like structure, solubility and pH values of different ORC were analyzed. The cytotoxicity was detected via DNA-binding fluorescence dye in Schwann cells, astrocytes and neuronal cells. Detachment of cells caused by ORC treatment was examined using crystal violet staining. Additionally, organotypic hippocampal slice cultures (OHSC) were treated for 24 and 48 hours with ORC. Propidium iodide, haematoxylin-eosin and isolectin B4 staining was used to analyze the cellular damage, cytoarchitecture and microglia activation.

Results: Cell death analysis showed a better compatibility of Equitamp® compared to Equicel® and Tabotamp® (p<0.001). Furthermore, Equitamp® treatment led to equivalent cytotoxicity of neuronal cells compared to the non-oxidized control material (cotton gauze). Additionally, OHSC damage was stronger after Tabotamp® and Equicel® treatment in comparison to Equitamp® or gauze (p<0.01). In contrast to the other ORC, Equicel® led to a very early and strong increase of microglia cells after 24 hours (p<0.001). Immunofluorescence staining was not detectable after Tabotamp® treatment, which might be due to an artifact caused by strongly reduced pH. In contrast to Tabotamp®, Equicel® displayed a neutral pH in aqueous solution but was not soluble within 48 hours. Equitamp® led to a less decreased pH of 4.8 at concentration of 500 mm2/ml (p<0.001) with a comparable solubility to Tabotamp®.

Conclusion: The application of Equitamp® resulted in significantly decreased damage and cell death of neuronal tissue compared to Equicel® and Tabotamp®. Therefore, ORC like Tabotamp® or Equicel® should be carefully used in eloquent areas.